微流控技术助力循环肿瘤细胞的分离与单细胞分析。
Microfluidics-Enabled Isolation and Single-Cell Analysis of Circulating Tumor Cells.
机构信息
Interdisciplinary Microsystems Group, Department of Mechanical and Aerospace Engineering, University of Florida, Gainesville, FL, USA.
Department of Pediatrics, Gainesville, FL, USA.
出版信息
Methods Mol Biol. 2023;2689:71-93. doi: 10.1007/978-1-0716-3323-6_7.
Microfluidic platforms enable the enrichment and analysis of circulating tumor cells (CTCs), a potential biomarker for cancer diagnosis, prognosis, and theragnosis. Combined with immunocytochemistry/immunofluorescence (ICC/IF) assays for CTCs, microfluidics-enabled detection presents a unique opportunity to study tumor heterogeneity and predict treatment response, both of which can help cancer drug development. In this chapter, we detail the protocols and methods employed to fabricate and use a microfluidic device for the enrichment, detection, and analysis of single CTCs from the blood samples of sarcoma patients.
微流控平台可用于富集和分析循环肿瘤细胞（CTC），这是癌症诊断、预后和治疗的潜在生物标志物。结合用于 CTC 的免疫细胞化学/免疫荧光（ICC/IF）检测，微流控技术能够进行检测，为研究肿瘤异质性和预测治疗反应提供了独特的机会，这两者都有助于癌症药物的开发。在本章中，我们详细介绍了制造和使用微流控装置从肉瘤患者的血液样本中富集、检测和分析单个 CTC 的方案和方法。
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