新型循环肿瘤相关细胞的发现对用于富集和检测的微流控装置提出了新的挑战。

The Discovery of Novel Circulating Cancer-Related Cells in Circulation Poses New Challenges to Microfluidic Devices for Enrichment and Detection.

机构信息

State Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai, 200050, China.

Center of Materials Science and Optoelectronics Engineering, University of Chinese Academy of Sciences, Beijing, 100049, China.

出版信息

Small Methods. 2022 Jul;6(7):e2200226. doi: 10.1002/smtd.202200226. Epub 2022 May 20.

DOI:10.1002/smtd.202200226

PMID:35595707

Abstract

Circulating tumor cells (CTCs) enumeration has been widely used as a surrogate predictive marker for early diagnoses, the evaluation of chemotherapy efficacy, and cancer prognosis. Microfluidic technologies for CTCs enrichment and detection have been developed and commercialized as automation platforms. Currently, in addition to CTCs, some new types of circulating cancer-related cells (e.g., CCSCs, CTECs, CAMLs, and heterotypic CTC clusters) in circulation are also reported to be correlated to cancer diagnosis, metastasis, or prognosis. And they widely differ from the conventional CTCs in positive markers, cellular morphology, or size, which presents a new technological challenge to microfluidic devices that use affinity-based capture methods or size-based filtration methods for CTCs detection. This review focuses on the biological and physical properties as well as clinical significance of the novel circulating cancer-related cells, and discusses the challenges of their discovery to microfluidic chip for enrichment. Finally, the current challenges of CTCs detection in clinical application and future opportunities are also discussed.

摘要

循环肿瘤细胞（CTC）的计数已被广泛用作早期诊断、化疗疗效评估和癌症预后的替代预测标志物。微流控技术已被开发和商业化用于 CTC 的富集和检测，作为自动化平台。目前，除了 CTC 之外，循环中还存在一些新型的与癌症相关的循环肿瘤细胞（如 CCSCs、CTECs、CAMLs 和异质 CTC 簇），也被报道与癌症的诊断、转移或预后相关。它们与传统的 CTCs 在阳性标志物、细胞形态或大小上有很大的不同，这给基于亲和力捕获或大小过滤方法的微流控设备检测 CTCs 带来了新的技术挑战。本综述重点介绍了新型循环肿瘤相关细胞的生物学和物理特性及其临床意义，并讨论了其在微流控芯片中发现的挑战，用于富集。最后，还讨论了 CTCs 在临床应用中的当前检测挑战和未来机遇。