1795年博氏黄鳝（鱼类，合鳃鱼科）血红蛋白和珠蛋白的特性研究

Characterization of the hemoglobins and globins of Synbranchus marmoratus Bloch, 1795 (Pisces, Synbranchidae).

作者信息

Nakamoto W, Machado P E

出版信息

Comp Biochem Physiol B. 1986;84(3):383-6. doi: 10.1016/0305-0491(86)90094-5.

Abstract

Total hemolysates of Synbranchus marmoratus Bloch, 1795, captured in Vitoriana, district of Botucatu, State of São Paulo, Brazil, were submitted to agar-starch gel electrophoresis on glass slides using 42 mM-Tris 1.7 mM EDTA-6.1 mM borate buffer, pH 8.8, for the gel and 10 mM borate-1.7 mM NaOH buffer, pH 8.6, for the cuvette. Three distinct hemoglobin bands were detected, with Hb I being of the cathodic type. Cellulose acetate electrophoresis in 800 mM Tris-2.1 mM EDTA buffer, pH 8.9, containing 6 M urea and 2.25 mM beta-mercaptoethanol indicated the presence of four globin chains denoted alpha 1, alpha 2, beta and gamma. It is suggested that the probable tetrameric constitution of the hemoglobin of Synbranchus marmoratus Bloch, 1795 is Hb I (alpha (2)2 gamma 2), Hb II (alpha (1)2 gamma 2) and Hb III (alpha (1)2 beta 2).

摘要

1795年在巴西圣保罗州博图卡图市维多利亚纳捕获的斑纹合鳃鱼（Synbranchus marmoratus Bloch）的全血溶血产物，使用42 mM - Tris 1.7 mM EDTA - 6.1 mM硼酸盐缓冲液（pH 8.8）作为凝胶缓冲液，10 mM硼酸盐 - 1.7 mM NaOH缓冲液（pH 8.6）作为比色杯缓冲液，在载玻片上进行琼脂 - 淀粉凝胶电泳。检测到三条不同的血红蛋白带，其中Hb I为阴极型。在含有6 M尿素和2.25 mMβ - 巯基乙醇的800 mM Tris - 2.1 mM EDTA缓冲液（pH 8.9）中进行醋酸纤维素电泳，结果表明存在四种球蛋白链，分别为α1、α2、β和γ。推测1795年斑纹合鳃鱼（Synbranchus marmoratus Bloch）血红蛋白的可能四聚体组成是Hb I（α(2)2γ2）、Hb II（α(1)2γ2）和Hb III（α(1)2β2）。