Srinivas Meghana, O'Sullivan Orla, Cotter Paul D, Sinderen Douwe van, Kenny John G
Food Biosciences Department, Teagasc Food Research Centre, Moorepark, P61 C996 Cork, Ireland.
APC Microbiome Ireland, University College Cork, T12 CY82 Cork, Ireland.
Foods. 2022 Oct 21;11(20):3297. doi: 10.3390/foods11203297.
The microbial communities present within fermented foods are diverse and dynamic, producing a variety of metabolites responsible for the fermentation processes, imparting characteristic organoleptic qualities and health-promoting traits, and maintaining microbiological safety of fermented foods. In this context, it is crucial to study these microbial communities to characterise fermented foods and the production processes involved. High Throughput Sequencing (HTS)-based methods such as metagenomics enable microbial community studies through amplicon and shotgun sequencing approaches. As the field constantly develops, sequencing technologies are becoming more accessible, affordable and accurate with a further shift from short read to long read sequencing being observed. Metagenomics is enjoying wide-spread application in fermented food studies and in recent years is also being employed in concert with synthetic biology techniques to help tackle problems with the large amounts of waste generated in the food sector. This review presents an introduction to current sequencing technologies and the benefits of their application in fermented foods.
发酵食品中存在的微生物群落多样且动态,会产生多种负责发酵过程的代谢产物,赋予发酵食品独特的感官品质和健康促进特性,并维持发酵食品的微生物安全性。在这种背景下,研究这些微生物群落对于表征发酵食品及其相关生产过程至关重要。基于高通量测序(HTS)的方法,如宏基因组学,能够通过扩增子测序和鸟枪法测序方法进行微生物群落研究。随着该领域的不断发展,测序技术变得越来越容易获得、价格合理且准确,并且正在进一步从短读长测序向长读长测序转变。宏基因组学在发酵食品研究中得到了广泛应用,近年来还与合成生物学技术协同使用,以帮助解决食品行业产生的大量废弃物问题。本综述介绍了当前的测序技术及其在发酵食品中应用的益处。