Department of Chemistry, University of North Bengal, Darjeeling 734013, India.
Department of Chemistry, Government General Degree College at Kushmandi, Dakshin Dinajpur, Kushmandi 733121, India.
Inorg Chem. 2023 Jul 24;62(29):11291-11303. doi: 10.1021/acs.inorgchem.3c00538. Epub 2023 Jul 11.
A biosensor comprising crystalline CuS nanoparticles (NPs) was synthesized via a one-step simple coprecipitation route without involvement of a surfactant. The powder X-ray diffraction method has been used to evaluate the crystalline nature and different phases consist of the formation of CuS NPs. Mainly hexagonal unit cells consist of the formation of CuS NP unit cells. Most of the surfaces are covered with rhombohedral microparticles with a smooth exterior and surface clustering, examined by SEM images, and the shape of NPs was spherical, having an average size of 23 nm, as confirmed by TEM analysis. This study has focused on the peroxidase-mimicking activity, superoxide dismutase (SOD)-mimicking activity, and chemosensor-based colorimetric determination and detection of epinephrine (EP) neurotransmitters with excellent selectivity. The CuS NPs catalyzed the oxidation of the oxidase substrate 3, 3-5, 5 tetramethyl benzidine (TMB) with the help of supplementary HO that followed Michaelis-Menten kinetics with excellent and values calculated by the Lineweaver-Burk plot. Taking advantage of the drop in absorbance upon introduction of EP for the CuS NPs-TMB/HO system, a colorimetric route has been developed for selective and real-time detection of EP. The sensitivity of the new colorimetric probe was vibrant, having a linear range of 0-16 μM, and achieved a low limit of detection of 457 nM. Moreover, the present nanosystem exhibited appreciable SOD-mimicking activity which could effectively remove O from commercial cigarette smoke, along with it acting as a potential radical scavenger as well. The new nanosystem effectively scavenged OH, O, and metal chelation which were investigated calorimetrically.
一种基于结晶 CuS 纳米粒子 (NPs) 的生物传感器通过一步简单的共沉淀法合成，无需使用表面活性剂。粉末 X 射线衍射法用于评估结晶性质和不同相的组成，形成 CuS NPs。主要的六方单元由 CuS NP 单元的形成组成。SEM 图像显示，大多数表面都覆盖着具有光滑外表面和表面团聚的菱面体微粒，通过 TEM 分析证实，NPs 的形状为球形，平均尺寸为 23nm。本研究主要集中在过氧化物酶模拟活性、超氧化物歧化酶 (SOD) 模拟活性、基于比色法的化学传感器以及对儿茶酚胺 (EP) 神经递质的选择性检测和检测。CuS NPs 在辅助 HO 的帮助下催化氧化酶底物 3,3-5,5 四甲基联苯胺 (TMB)，遵循 Michaelis-Menten 动力学，通过 Lineweaver-Burk 图计算得出的 和 值非常好。利用引入 EP 后 CuS NPs-TMB/HO 体系吸光度的下降，开发了一种用于选择性和实时检测 EP 的比色法。新比色探针的灵敏度很高，线性范围为 0-16 μM，检测限达到 457 nM。此外，该纳米系统表现出可观的 SOD 模拟活性，能够有效清除商业香烟烟雾中的 O，同时作为一种潜在的自由基清除剂。新的纳米系统通过量热法有效地清除了 OH、O 和金属螯合。
