Food safety is a concerning issue globally. Foodborne-pathogenic-bacteria-derived foodborne disease outbreaks have increased the threat to human health. The accurate and rapid detection of foodborne bacteria is of great significance for food safety. A fiber-optic-based biosensor has emerged as a powerful technique for the point-of-care testing of foodborne bacteria in food and agricultural products. This Perspective discusses the opportunities and challenges of fiber-optic-based biosensors for foodborne bacteria detection. The corresponding solution strategies to promote the application of this innovative technology in food and agricultural product detection for food safety and human health are also discussed and proposed.