Ran Lu, Feng Niu, Dong Yiming, Cai Huanyu, Chen Yiping, Teng Huailong
College of Chemistry, Huazhong Agricultural University, Wuhan, 430070, China.
Hubei Key Laboratory of Natural Products Research and Development, College of Biological and Pharmaceutical Sciences, China Three Gorges University, Yichang, 443002, China.
Adv Sci (Weinh). 2025 Jun;12(22):e2414866. doi: 10.1002/advs.202414866. Epub 2025 May 14.
Food safety have received increasing attention in recent years, and rapid detection and thorough removal of organic contaminants is an important part of food safety control. In this work, a novel multi-functional photo-enzymatic nanoreactor HRP@Fe-NU-1003 is developed through the co-immobilization of horseradish peroxidase (HRP) and FeCl on a photosensitive metal-organic frameworks (MOF) NU-1003. The bio-nanocluster can serve as an efficient biosensor in the detection of chloramphenicol (CAP), with a detection limit of 15.38 pg mL, which is 62 times greater than that of the conventional HRP- enzyme-linked immunosorbent assay method. Besides its detecting capability, the nanoreactor also exhibits high efficiency in the photocatalytic degradation of CAP, which can remove 50 µg mL of CAP thoroughly within 30 min, and the mineralization efficiency of CAP reaches 61%. In this material, Fe-NU-1003 not only acts as a protecting shell to prevent HRP from deactivation, but improves detecting sensitivity and photocatalytic performance. Mechanism studies show that FeCl enhances its photocatalytic performance through promoting electron (e)-hole (h) separation and photocurrent transfer. More importantly, the heterogeneous material possesses high stability and can be recycled at least five rounds while its photocatalytic performance maintained at a high level. This strategy provides a new approach for the detection and degradation of pollutants.
近年来，食品安全受到了越来越多的关注，快速检测和彻底去除有机污染物是食品安全控制的重要组成部分。在这项工作中，通过将辣根过氧化物酶（HRP）和FeCl共固定在光敏金属有机框架（MOF）NU-1003上，开发了一种新型多功能光酶纳米反应器HRP@Fe-NU-1003。该生物纳米簇可作为检测氯霉素（CAP）的高效生物传感器，检测限为15.38 pg/mL，比传统的HRP酶联免疫吸附测定法高62倍。除了检测能力外，该纳米反应器在CAP的光催化降解方面也表现出高效性，可在30分钟内彻底去除50 μg/mL的CAP，CAP的矿化效率达到61%。在这种材料中，Fe-NU-1003不仅作为保护壳防止HRP失活，还提高了检测灵敏度和光催化性能。机理研究表明，FeCl通过促进电子（e）-空穴（h）分离和光电流转移来增强其光催化性能。更重要的是，这种异质材料具有高稳定性，可循环使用至少五轮，同时其光催化性能保持在高水平。该策略为污染物的检测和降解提供了一种新方法。
