Shanghai Engineering Research Center of Food Microbiology, School of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China.
Shanghai Engineering Research Center of Food Microbiology, School of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China.
Food Res Int. 2024 Oct;193:114767. doi: 10.1016/j.foodres.2024.114767. Epub 2024 Jul 29.
In recent years, foodborne diseases have posed a serious threat to human health, and rapid detection of foodborne pathogens is particularly crucial for the prevention and control of such diseases. This article offers a detailed overview of the development of detection techniques for foodborne pathogens, transitioning from traditional microbiological culture methods to the current array of techniques, including immunological, molecular biological, and biosensor-based methods. It summarizes the technical principles, advantages, disadvantages, and research progress of these diverse methods. Furthermore, the article demonstrates that the combination of different methods enhances the efficiency and accuracy of pathogens detection. Specifically, the article focuses on the application and advantages of combining CRISPR/Cas systems with other detection methods in the detection of foodborne pathogens. CRISPR/Cas systems, with their high specificity, sensitivity, and ease of operation, show great potential in the field of foodborne pathogens detection. When integrated with other detection techniques such as immunological detection techniques, molecular biology detection techniques, and biosensors, the accuracy and efficiency of detection can be further improved. By fully utilizing these tools, early detection and control of foodborne diseases can be achieved, enhancing public health and preventing disease outbreaks. This article serves as a valuable reference for exploring more convenient, accurate, and sensitive field detection methods for foodborne pathogens, promoting the application of rapid detection techniques, and ensuring food safety and human health.
近年来，食源性疾病对人类健康构成了严重威胁，快速检测食源性致病菌对于此类疾病的预防和控制尤为关键。本文详细介绍了食源性致病菌检测技术的发展，从传统的微生物培养方法发展到目前包括免疫学、分子生物学和基于生物传感器的方法在内的多种技术。本文总结了这些不同方法的技术原理、优缺点和研究进展。此外，文章还表明，不同方法的结合可以提高致病菌检测的效率和准确性。具体而言，本文重点介绍了 CRISPR/Cas 系统与其他检测方法在食源性致病菌检测中的应用和优势。CRISPR/Cas 系统具有特异性高、灵敏度高、操作简便等优点，在食源性致病菌检测领域具有很大的应用潜力。当与免疫学检测技术、分子生物学检测技术和生物传感器等其他检测技术结合使用时，可以进一步提高检测的准确性和效率。通过充分利用这些工具，可以实现食源性疾病的早期检测和控制，提高公众健康水平，预防疾病爆发。本文为探索更方便、准确、灵敏的食源性致病菌现场检测方法提供了有价值的参考，促进了快速检测技术的应用，保障了食品安全和人类健康。
