College of Food Science, Southwest University, Chongqing 400715, China.
College of Pharmaceutical Sciences, Southwest University, Chongqing 400715, China.
Food Chem. 2023 Dec 1;428:136774. doi: 10.1016/j.foodchem.2023.136774. Epub 2023 Jul 6.
In this work, a lateral flow assay for Listeria monocytogenes was developed based on phage tail fiber protein (TFP) and triple-functional nanozyme probes with capture-separation-catalytic activity. Inspired by interaction between phage and bacteria, TFP of L. monocytogenes phage was immobilized on test line as capture molecule, which replaced traditional antibody and aptamer. After Gram-positive bacteria was captured and separated from samples by nanozyme probes modified with vancomycin (Van), TFP specifically recognized L. monocytogenes and overcame non-specific binding of Van. Special color reaction between Coomassie Brilliant Blue and bovine serum albumin which was an amplification carrier on probe was simply utilized as control zone to replace traditional control line. Relying on enzyme-like catalytic activity of nanozyme, this biosensor realized improved sensitivity and colorimetric quantitative detection with a detection limit of 10 CFU mL. Analytic performance results suggested this TFP-based biosensor provided a portable, sensitive and specific strategy to detect pathogen.
在这项工作中，基于噬菌体尾纤维蛋白（TFP）和具有捕获-分离-催化活性的多功能纳米酶探针，开发了一种用于李斯特菌的侧向流动检测法。受噬菌体与细菌相互作用的启发，将李斯特菌噬菌体的 TFP 固定在测试线上作为捕获分子，取代了传统的抗体和适体。纳米酶探针经万古霉素（Van）修饰后，可捕获和分离样品中的革兰氏阳性菌，然后 TFP 特异性识别李斯特菌，克服了 Van 的非特异性结合。探针上的放大载体牛血清白蛋白和考马斯亮蓝之间的特殊显色反应被简单用作对照线，以替代传统的对照线。该生物传感器依赖于纳米酶的类酶催化活性，实现了改进的灵敏度和比色定量检测，检测限为 10 CFU mL。分析性能结果表明，这种基于 TFP 的生物传感器为检测病原体提供了一种便携、灵敏和特异的策略。
