State Key Laboratory of Food Science and Resources, Nanchang University, Nanchang 330047, PR China; International Institute of Food Innovation Co., Ltd., Nanchang University, Nanchang 330200, PR China.
State Key Laboratory of Food Science and Resources, Nanchang University, Nanchang 330047, PR China.
Food Chem. 2025 Jan 15;463(Pt 4):141495. doi: 10.1016/j.foodchem.2024.141495. Epub 2024 Sep 30.
For foodborne pathogens pose a serious threat to public health, a magnetic separation strategy and a nanozyme-based biosensor are proposed for biosensing of Listeria monocytogenes (L. monocytogenes). In this work, doripenem is selected as a recognized molecule for the modification of magnetic beads to capture L.monocytogenes in food and environmental samples. Furthermore, the POD-like MXene-Hemin-Au is constructed from perspective of charge transfer engineering which provides a vivid example to rational design of nanozymes. Finally, the captured L.monocytogenes is labeled with MXene-Hemin-Au@mAb, forming the sandwich complexes for quantitative determination. The current signals that generated by the complexes exhibit a good linear relationship with a limit of detection of 2.3 × 10 CFU/mL. The biosensor shows a satisfactory applicability in real samples with recoveries of 91.19% to 102.98%. Overall, the biosensor with integrated magnetic separation strategy presents a potential approach for high sensitivity biosensing of foodborne pathogens.
由于食源性病原体严重威胁公众健康，因此提出了一种磁分离策略和一种基于纳米酶的生物传感器，用于检测单核细胞增生李斯特菌（L. monocytogenes）。在这项工作中，选择多利培南作为修饰磁珠的公认分子，以捕获食品和环境样本中的单核细胞增生李斯特菌。此外，从电荷转移工程的角度构建了具有过氧化物酶样活性的 MXene-血红素-Au，为纳米酶的合理设计提供了一个生动的例子。最后，将捕获的单核细胞增生李斯特菌用 MXene-血红素-Au@mAb 标记，形成用于定量测定的三明治复合物。复合物产生的电流信号与检测限为 2.3×10 CFU/mL 呈现出良好的线性关系。该生物传感器在实际样品中的应用表现出令人满意的回收率为 91.19%至 102.98%。总体而言，具有集成磁分离策略的生物传感器为食源性病原体的高灵敏度生物传感提供了一种潜在的方法。
