Liu C J, Wang Z Q, Chang J W, Zhang X, Liu X B, Liu C N, Ma T X
Zhonghua Zhong Liu Za Zhi. 1986 May;8(3):178-80.
Thirty specimens from 30 patients with urologic and male genital system tumors were cultured. Eighteen (60%) showed initial cell growth. Four (13%) were successful in primary culture. In a renal cell carcinoma and a bladder transitional cell carcinoma, the subcultures were successful after the primary culture. In eight months, having transferred the former for twelve generations and the latter for twenty-seven generations, these two cell lines are established. The morphological characters of the cultured cells, eradication of fibroblasts, conditions of culture and prevention of contamination are discussed.
对30例泌尿系统和男性生殖系统肿瘤患者的30个标本进行了培养。18个（60%）显示出初始细胞生长。4个（13%）原代培养成功。在1例肾细胞癌和1例膀胱移行细胞癌中，原代培养后传代培养成功。在8个月内，将前者传代12次，后者传代27次，建立了这两种细胞系。文中讨论了培养细胞的形态特征、成纤维细胞的清除、培养条件及污染的预防。
