STING-IFN-I 通路通过抑制大鼠背根神经节中的神经炎症来缓解切口诱导的急性术后疼痛。
STING-IFN-I pathway relieves incision induced acute postoperative pain via inhibiting the neuroinflammation in dorsal root ganglion of rats.
机构信息
Department of Anesthesiology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China.
Department of Anesthesiology, Zhongnan Hospital of Wuhan University, Wuhan, 430071, China.
出版信息
Inflamm Res. 2023 Aug;72(8):1551-1565. doi: 10.1007/s00011-023-01764-6. Epub 2023 Jul 11.
BACKGROUND
The purpose of this study was to study the effect of STING-IFN-I pathway on incision induced postoperative pain in rats and its possible mechanisms.
METHODS
The pain thresholds were evaluated by measuring the mechanical withdrawal threshold and the thermal withdrawal latency. The satellite glial cell and macrophage of DRG were analyzed. The expression of STING, IFN-a, P-P65, iNOS, TNF-α, IL-1β and IL-6 in DRG was evaluated.
RESULTS
The activation of STING-IFN-I pathway can reduce the mechanical hyperalgesia, thermal hyperalgesia, down-regulate the expression of P-P65, iNOS, TNF-α, IL-1β and IL-6, and inhibit the activation of satellite glial cell and macrophage in DRG.
CONCLUSIONS
The activation of STING-IFN-I pathway can alleviate incision induced acute postoperative pain by inhibiting the activation of satellite glial cell and macrophage, which reducing the corresponding neuroinflammation in DRG.
背景
本研究旨在探讨 STING-IFN-I 通路对大鼠切口诱导术后疼痛的影响及其可能的机制。
方法
通过测量机械撤足阈值和热撤足潜伏期来评估疼痛阈值。分析 DRG 中的卫星胶质细胞和巨噬细胞。评估 DRG 中 STING、IFN-α、P-P65、iNOS、TNF-α、IL-1β 和 IL-6 的表达。
结果
STING-IFN-I 通路的激活可减轻机械性痛觉过敏、热痛觉过敏，下调 P-P65、iNOS、TNF-α、IL-1β 和 IL-6 的表达，并抑制 DRG 中卫星胶质细胞和巨噬细胞的激活。
结论
STING-IFN-I 通路的激活通过抑制卫星胶质细胞和巨噬细胞的激活，减轻 DRG 中的相应神经炎症，从而缓解切口诱导的急性术后疼痛。
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