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外源丙酮 - (2-萘基磺酰基)肟通过玉米中的铜解毒系统改善过量铜的不良反应。

Exogenous acetone -(2-naphthylsulfonyl)oxime improves the adverse effects of excess copper by copper detoxification systems in maize.

机构信息

Department of Plant and Animal Production, Muş Alparslan University, Muş, Türkiye.

出版信息

Int J Phytoremediation. 2023;25(14):2001-2013. doi: 10.1080/15226514.2023.2234489. Epub 2023 Jul 11.

Abstract

The current study is to elucidate the responses of maize seedlings to excess copper and acetone -(2-naphthylsulfonyl)oxime (NS) pretreatment. The study was divided into the following experimental groups: 18 h distilled water (DW) control (C), 6 h 0.3 mM NS + then 12 h DW (NS), 6 h DW + then 12 h 1 mM CuSO.5HO (CuS), 0.3 mM NS for 6 h + then 1 mM CuSO.5HO (NS + CuS) for 12 h. When the NS + CuS group is compared with the CuS group; It accumulated 10% more copper, while the ABA, HO, MDA, and carotenoid contents decreased significantly, the total chlorophyll, proline, gallic acid, ascorbic acid, catechol, trans-P-qumaric acid, and cinnamic acid contents increased. While SOD activity, which is one of the antioxidant system enzymes, decreased with NS application, GPX, CAT, and APX activities increased despite copper stress. When all the findings are evaluated as a whole, exogenous NS, despite excessive copper, ameliorated the adverse effects of copper stress by increasing the effectiveness of the enzymatic and non-enzymatic components of the antioxidant system and the contents of phenolic substances. In addition, increasing the copper content by 10% reveals its importance in terms of NS phytoremediation. Style-sheet: When full form and abbreviated form both are used as keywords, retain both as provided by the author.

摘要

本研究旨在阐明玉米幼苗对过量铜和丙酮-(2-萘基磺酰基)肟(NS)预处理的反应。该研究分为以下实验组:18 小时蒸馏水(DW)对照(C)、6 小时 0.3 mM NS+然后 12 小时 DW(NS)、6 小时 DW+然后 12 小时 1 mM CuSO.5HO(CuS)、6 小时 0.3 mM NS+然后 12 小时 1 mM CuSO.5HO(NS+CuS)。与 CuS 组相比,当 NS+CuS 组与 CuS 组相比时,其铜积累量增加了 10%,而 ABA、HO、MDA 和类胡萝卜素含量显著下降,总叶绿素、脯氨酸、没食子酸、抗坏血酸、儿茶酚、反式-P-奎马酸和肉桂酸含量增加。虽然 NS 应用会降低抗氧化系统酶之一的 SOD 活性,但 GPX、CAT 和 APX 活性增加,尽管存在铜胁迫。当将所有发现作为一个整体进行评估时,外源性 NS 尽管存在过量的铜,但通过增加抗氧化系统的酶和非酶成分以及酚类物质的含量,改善了铜胁迫的不利影响。此外,将铜含量增加 10% 表明其在 NS 植物修复方面的重要性。

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