Department of Medical Oncology, Fujian Medical University Union Hospital, Fuzhou, Fujian Province, China.
Department of Medical Oncology, Zhangzhou Municipal Hospital, Zhangzhou, Fujian Province, China.
Turk J Gastroenterol. 2023 Sep;34(9):975-981. doi: 10.5152/tjg.2023.23108.
BACKGROUND/AIMS: Transforming growth factor-beta can influence tumor cells, causing epithelial-mesenchymal transition and enhancing their invasion and metastasis ability. Rac1 protein could be used as an independent tumor diagnostic marker and survival predictor. Prex1 is closely related to cell metastasis. In this study, the impact of silencing Rac1 and Prex1 on transforming growth factor-beta 1-induced epithelial-mesenchymal transition and apoptosis of human gastric cancer cells MGC-803 and MKN45 was investigated.
MGC-803 and MKN45 cells received recombinant transforming growth factor-beta 1 (rTGF-β1) treatments at various concentrations. Cell Counting Kit-8 kit was used to determine cell viability. Rac1 and Prex1 interference vectors were transfected into the rTGF-β1-treated MGC-803 and MKN45 cells. Cell apoptosis and migration were detected by flow cytometry and scratch test, respectively. Western blot was used to detect the epithelial-mesenchymal transition-related markers E-cadherin, N-cadherin, vimentin, and PDLIM2 expression levels.
The rTGF-β1 (10 ng/mL) could promote MGC-803 and MKN45 cell viability. Silencing Rac1 and Prex1 could increase E-cadherin and PDLIM2 expression, decrease N-cadherin and vimentin expression, inhibit cell viability and migration, and promote apoptosis in rTGF-β1-treated MGC-803 and MKN45 cells.
Silencing Rac1 and Prex1 could inhibit epithelial-mesenchymal transition, reduce cell viability and migration, and promote apoptosis in human gastric cancer cells.
背景/目的：转化生长因子-β 可影响肿瘤细胞，导致上皮-间充质转化，增强其侵袭和转移能力。Rac1 蛋白可作为独立的肿瘤诊断标志物和生存预测因子。Prex1 与细胞转移密切相关。本研究探讨了沉默 Rac1 和 Prex1 对转化生长因子-β 1 诱导的人胃癌细胞 MGC-803 和 MKN45 上皮-间充质转化和凋亡的影响。
用不同浓度的重组转化生长因子-β 1（rTGF-β1）处理 MGC-803 和 MKN45 细胞。用细胞计数试剂盒-8 检测细胞活力。将 Rac1 和 Prex1 干扰载体转染到 rTGF-β1 处理的 MGC-803 和 MKN45 细胞中。用流式细胞术检测细胞凋亡，划痕试验检测细胞迁移。用 Western blot 检测上皮-间充质转化相关标志物 E-钙黏蛋白、N-钙黏蛋白、波形蛋白和 PDLIM2 的表达水平。
rTGF-β1（10ng/mL）可促进 MGC-803 和 MKN45 细胞活力。沉默 Rac1 和 Prex1 可增加 E-钙黏蛋白和 PDLIM2 的表达，降低 N-钙黏蛋白和波形蛋白的表达，抑制 rTGF-β1 处理的 MGC-803 和 MKN45 细胞的活力和迁移，促进细胞凋亡。
沉默 Rac1 和 Prex1 可抑制人胃癌细胞的上皮-间充质转化，降低细胞活力和迁移，促进细胞凋亡。
