Zangeneh Motlagh Melika, Mahdavi Nazanin, Miri-Lavasani Zohreh, Aminishakib Pouyan, Khoramipour Mahsa, Kazem Arki Mandana, Mirlavasni Zohreh, Rezaei Niloufar, Hossein-Khannazer Nikoo, Vosough Massoud
Department of Oral and Maxillofacial Pathology, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran.
Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Cell J. 2023 Jun 28;25(6):407-417. doi: 10.22074/cellj.2023.1985869.1202.
Surgery and chemotherapy are the most common therapeutic strategies proposed for oral squamous cell carcinoma (OSCC). However, some of the disadvantages associated with the current methods like unwanted side effects and poor drug response lead the scientist to seek for novel modalities and delivery approaches to enhance the efficacy of treatments. The study aimed to assess the effectiveness of disulfiram (DSF)-loaded Niosomes on cancerous phenotypes of the OSCC cells.
In this experimental study, an optimum formulation of DSF-loaded Niosomes was developed for the treatment of OSCC cells to reduce drug doses and improve the poor stability of DSF in the OSCC environment. The design expert software was utilized to optimize the particles in terms of size, polydispersity index (PDI), and entrapment efficacy (EE).
Acidic pH increased the release rate of DSF from these formulations. The size, PDI, and EE of Niosomes were more stable at 4°C compared to 25°C. The results indicated that DSF-loaded Niosomes could induce apoptosis (P=0.019) in the OSCC cells compared to the control group. Moreover, it could reduce colony formation ability (P=0.0046) and also migration capacity of OSCC cells (P=0.0015).
Our findings indicated that the application of proper dose of DSF-loaded Niosomes (12.5 μg/ml) increases apoptosis, decreases colony formation capacity and declines the migration ability of OSCC cells.
手术和化疗是针对口腔鳞状细胞癌（OSCC）提出的最常见治疗策略。然而，当前方法存在一些缺点，如不良副作用和药物反应不佳，这促使科学家寻求新的治疗方式和给药途径，以提高治疗效果。本研究旨在评估载有双硫仑（DSF）的脂质体对OSCC细胞癌性表型的有效性。
在本实验研究中，开发了一种载有DSF的脂质体的最佳配方，用于治疗OSCC细胞，以减少药物剂量并改善DSF在OSCC环境中的稳定性差的问题。利用设计专家软件在尺寸、多分散指数（PDI）和包封率（EE）方面对颗粒进行优化。
酸性pH值增加了DSF从这些制剂中的释放速率。与25°C相比，脂质体的尺寸、PDI和EE在4°C时更稳定。结果表明，与对照组相比，载有DSF的脂质体可诱导OSCC细胞凋亡（P = 0.019）。此外，它还可以降低OSCC细胞的集落形成能力（P = 0.0046）和迁移能力（P = 0.0015）。
我们的研究结果表明，应用适当剂量的载有DSF的脂质体（12.5μg/ml）可增加凋亡、降低集落形成能力并降低OSCC细胞的迁移能力。
