Student Research Committee, Faculty of Dentistry, Aja University of Medical Sciences, Tehran, Iran.
Dental Research Centre, Oral Pathology Department, Dental Faculty, Hamadan University of Medical Sciences, Hamadan, Iran.
BMC Complement Med Ther. 2022 Apr 19;22(1):109. doi: 10.1186/s12906-022-03590-5.
Oral malignancies have a significant effect on the quality of life among the affected patients. Curcumin is an antioxidant with a low bioavailability in the target tissue. Niosomes are carriers of increasing the therapeutic effects of drugs and reducing their side effects. This study aimed to determine the effective dose of curcumin niosome in the culture and then to compare its prophylactic effect in the form of mouthwash with that of its injectable form on oral cancer in rats.
This was an in-vitro and in-vivo study. Firstly, KB oral cancer cells and human umbilical vein endothelial cells (HUVEC) were treated in separate groups with free curcumin, curcumin-loaded niosomes, and the unloaded niosomes at four doses of 4, 8, 16, and 32 μg. The study rats were then divided into the following four groups: 1) no intervention, 2) only carcinogenic substance, 3) carcinogenic substance with curcumin-loaded niosome injection, and 4) carcinogenic substance with a mouthwash containing niosome.
At the cellular level, a dose of 16 μg after 24 h was selected as an effective dose. In the animal phase, the use of injectable curcumin niosome was observed to significantly prevent the development of severe forms of dysplasia.
In this in-vitro and in-vivo study, curcumin-loaded niosome was effective in preventing the development of severe forms of dysplasia and the inhibition of the growth of cancer cells.
口腔恶性肿瘤对患者的生活质量有重大影响。姜黄素是一种抗氧化剂，在靶组织中的生物利用度较低。非离子表面活性剂囊泡是增加药物治疗效果和降低其副作用的载体。本研究旨在确定姜黄素非离子表面活性剂囊泡的有效剂量，然后比较其以漱口液形式和注射形式对大鼠口腔癌的预防作用。
这是一项体内外研究。首先，将 KB 口腔癌细胞和人脐静脉内皮细胞（HUVEC）分别在 4、8、16 和 32μg 的四个剂量的游离姜黄素、载姜黄素非离子表面活性剂囊泡和未载药的非离子表面活性剂囊泡中进行处理。然后，将研究大鼠分为以下四组：1）无干预，2）仅致癌物质，3）载姜黄素非离子表面活性剂囊泡注射致癌物质，4）含非离子表面活性剂囊泡的漱口水漱口致癌物质。
在细胞水平上，选择 24 小时后 16μg 的剂量作为有效剂量。在动物阶段，观察到注射用姜黄素非离子表面活性剂囊泡的使用可显著预防重度发育不良的发生。
在这项体内外研究中，载姜黄素非离子表面活性剂囊泡可有效预防重度发育不良的发生和癌细胞的生长抑制。
