Li Jia, Cai Bo, Chang Sheng, Yang Ying, Zi Shuhui, Liu Tao
College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming, China.
National & Local Joint Engineering Research Center on Germplasm Innovation & Utilization of Chinese Medicinal Materials in Southwestern China, Kunming, China.
Front Plant Sci. 2023 Jun 26;14:1195932. doi: 10.3389/fpls.2023.1195932. eCollection 2023.
Tobacco black shank (TBS), caused by , is one of the most harmful diseases of tobacco. There are many studies have examined the mechanism underlying the induction of disease resistance by arbuscular mycorrhizal fungi (AMF) and β-aminobutyric acid (BABA) alone, but the synergistic effects of AMF and BABA on disease resistance have not yet been studied. This study examined the synergistic effects of BABA application and AMF inoculation on the immune response to TBS in tobacco. The results showed that spraying BABA on leaves could increase the colonization rate of AMF, the disease index of tobacco infected by treated with AMF and BABA was lower than that of alone. The control effect of AMF and BABA on tobacco infected by was higher than that of AMF or BABA and alone. Joint application of AMF and BABA significantly increased the content of N, P, and K in the leaves and roots, in the joint AMF and BABA treatment than in the sole treatment. The dry weight of plants treated with AMF and BABA was 22.3% higher than that treated with alone. In comparison to alone, the combination treatment with AMF and BABA had increased Pn, Gs, Tr, and root activity, while alone had reduced Ci, HO content, and MDA levels. SOD, POD, CAT, APX, and activity and expression levels were increased under the combined treatment of AMF and BABA than in alone. In comparison to the treatment of alone, the combined use of AMF and BABA increased the accumulation of GSH, proline, total phenols, and flavonoids. Therefore, the joint application of AMF and BABA can enhance the TBS resistance of tobacco plants to a greater degree than the application of either AMF or BABA alone. In summary, the application of defense-related amino acids, combined with inoculation with AMF, significantly promoted immune responses in tobacco. Our findings provide new insights that will aid the development and use of green disease control agents.
烟草黑胫病（TBS）由[病原体名称缺失]引起，是烟草最具危害性的病害之一。已有许多研究单独考察了丛枝菌根真菌（AMF）和β-氨基丁酸（BABA）诱导抗病性的潜在机制，但AMF与BABA对抗病性的协同效应尚未得到研究。本研究考察了施用BABA和接种AMF对烟草抗TBS免疫反应的协同效应。结果表明，在叶片上喷施BABA可提高AMF的定殖率，AMF和BABA处理的烟草感染[病原体名称缺失]后的病情指数低于单独使用AMF处理的。AMF和BABA对感染[病原体名称缺失]的烟草的防治效果高于单独使用AMF或BABA以及单独使用[病原体名称缺失]处理的。AMF和BABA联合施用显著增加了叶片和根系中N、P和K的含量，在AMF和BABA联合处理中的含量高于单独使用[病原体名称缺失]处理。AMF和BABA处理的植株干重比单独使用[病原体名称缺失]处理的高22.3%。与单独使用[病原体名称缺失]相比，AMF和BABA联合处理提高了净光合速率（Pn）、气孔导度（Gs）、蒸腾速率（Tr）和根系活力，而单独使用[病原体名称缺失]处理降低了胞间二氧化碳浓度（Ci）、过氧化氢（HO）含量和丙二醛（MDA）水平。在AMF和BABA联合处理下，超氧化物歧化酶（SOD）、过氧化物酶（POD）、过氧化氢酶（CAT）、抗坏血酸过氧化物酶（APX）和[酶名称缺失]的活性及表达水平高于单独使用[病原体名称缺失]处理。与单独使用[病原体名称缺失]处理相比，AMF和BABA联合使用增加了谷胱甘肽（GSH）、脯氨酸、总酚和类黄酮的积累。因此，AMF和BABA联合施用比单独施用AMF或BABA能更大程度地增强烟草植株对TBS的抗性。总之，施用与防御相关的氨基酸并结合接种AMF，显著促进了烟草的免疫反应。我们的研究结果提供了新的见解，将有助于绿色病害防治剂的开发和应用。
