Aleksic Dubravka, Poleksic Joko, Agatonovic Gorana, Djulejic Vuk, Vulovic Maja, Aksic Miljana, Reiss Gebhard, Hamad Mohammad I K, Jakovcevski Igor, Aksic Milan
School of Medicine, Institute of Anatomy "Niko Miljanić", University of Belgrade, Belgrade, Serbia.
Department of Anatomy, Faculty of Medical Sciences, University of Kragujevac, Kragujevac, Serbia.
Front Neurosci. 2023 Jun 26;17:1187758. doi: 10.3389/fnins.2023.1187758. eCollection 2023.
There is an increasing evidence supporting the hypothesis that traumatic experiences during early developmental periods might be associated with psychopathology later in life. Maternal deprivation (MD) in rodents has been proposed as an animal model for certain aspects of neuropsychiatric disorders.
To determine whether early-life stress leads to changes in GABAergic, inhibitory interneurons in the limbic system structures, specifically the amygdala and nucleus accumbens, 9-day-old Wistar rats were exposed to a 24 h MD. On postnatal day 60 (P60), the rats were sacrificed for morphometric analysis and their brains were compared to the control group.
Results show that MD affect GABAergic interneurons, leading to the decrease in density and size of the calcium-binding proteins parvalbumin-, calbindin-, and calretinin-expressing interneurons in the amygdala and nucleus accumbens.
This study indicates that early stress in life leads to changes in the number and morphology of the GABAergic, inhibitory interneurons in the amygdala and nucleus accumbens, most probably due to the loss of neurons during postnatal development and it further contributes to understanding the effects of maternal deprivation on brain development.
越来越多的证据支持这样一种假说，即早期发育阶段的创伤经历可能与日后生活中的精神病理学有关。啮齿动物的母爱剥夺（MD）已被提议作为神经精神疾病某些方面的动物模型。
为了确定早期生活压力是否会导致边缘系统结构，特别是杏仁核和伏隔核中γ-氨基丁酸能抑制性中间神经元发生变化，将9日龄的Wistar大鼠暴露于24小时的母爱剥夺环境中。在出生后第60天（P60），处死大鼠进行形态计量分析，并将其大脑与对照组进行比较。
结果表明，母爱剥夺会影响γ-氨基丁酸能中间神经元，导致杏仁核和伏隔核中表达钙结合蛋白小白蛋白、钙结合蛋白和钙视网膜蛋白的中间神经元密度和大小降低。
本研究表明，生命早期的压力会导致杏仁核和伏隔核中γ-氨基丁酸能抑制性中间神经元的数量和形态发生变化，这很可能是由于出生后发育过程中神经元的丢失所致，它进一步有助于理解母爱剥夺对大脑发育的影响。
