Faculty of Sport and Physical Education, University of Belgrade, Blagoja Parovića 156, Belgrade, Serbia.
Department of Psychiatry, University of Connecticut School of Medicine, Farmington, CT, USA.
Oxid Med Cell Longev. 2017;2017:7390516. doi: 10.1155/2017/7390516. Epub 2017 Mar 20.
Maternal deprivation (MD) causes perinatal stress, with subsequent behavioral changes which resemble the symptoms of schizophrenia. The NADPH oxidase is one of the major generators of reactive oxygen species, known to play a role in stress response in different tissues. The aim of this study was to elucidate the long-term effects of MD on the expression of NADPH oxidase subunits (gp91, p22, p67, p47, and p40). Activities of cytochrome C oxidase and respiratory chain Complex I, as well as the oxidative stress parameters using appropriate spectrophotometric techniques were analyzed. Nine-day-old Wistar rats were exposed to a 24 h maternal deprivation and sacrificed at young adult age. The structures affected by perinatal stress, cortex, hippocampus, thalamus, and caudate nuclei were investigated. The most prominent findings were increased expressions of gp91 in the cortex and hippocampus, increased expression of p22 and p40, and decreased expression of gp91, p22, and p47 in the caudate nuclei. Complex I activity was increased in all structures except cortex. Content of reduced glutathione was decreased in all sections while region-specific changes of other oxidative stress parameters were found. Our results indicate the presence of long-term redox alterations in MD rats.
母体剥夺（MD）导致围产期应激，随后出现类似精神分裂症症状的行为改变。NADPH 氧化酶是活性氧的主要产生者之一，已知在不同组织的应激反应中发挥作用。本研究旨在阐明 MD 对 NADPH 氧化酶亚基（gp91、p22、p67、p47 和 p40）表达的长期影响。使用适当的分光光度技术分析细胞色素 C 氧化酶和呼吸链复合物 I 的活性以及氧化应激参数。将 9 天大的 Wistar 大鼠暴露于 24 小时的母体剥夺中，并在成年早期处死。研究了受围产期应激影响的结构，包括皮质、海马体、丘脑和尾状核。最显著的发现是皮质和海马体中 gp91 的表达增加，尾状核中 p22 和 p40 的表达增加，以及 gp91、p22 和 p47 的表达减少。除皮质外，所有结构中的复合物 I 活性均增加。所有部位的还原型谷胱甘肽含量均降低，而其他氧化应激参数则存在特定区域的变化。我们的结果表明，MD 大鼠存在长期的氧化还原改变。
