Sindhu Haseeb Akram, Afzal Muhammad, Shahid Izzah
Department of Basic and Applied Chemistry, Faculty of Science and Technology, University of Central Punjab, Lahore, Pakistan.
Department of Biotechnology, Faculty of Science and Technology, University of Central Punjab, Lahore, Pakistan.
Dose Response. 2023 Jul 5;21(3):15593258231187357. doi: 10.1177/15593258231187357. eCollection 2023 Jul-Sep.
Medicinal plants have been extensively exploited for their immense pharmacological and immune-supporting potential. Fruit of has several active secondary metabolites such as phenolics, flavonoids, and essential oils that are used in traditional medicines as antidiabetic, anti-inflammatory, antioxidant, and antimicrobial agents. In this study, phytoconstituents in organic fractions (-hexane, chloroform, and ethyl acetate) of the methanolic extract of were analyzed and identified by FT-IR, HPLC, and GC-MS analysis. Ethyl acetate fraction showed the highest antioxidant scavenging (76 ± .769%) and anti-inflammatory (40 ± .473%) activities at the concentration of 3 mg/mL. Similarly, antidiabetic effect was measured by inhibition of α-amylase where, ethyl acetate fraction (77 ± .844%) exhibited the highest antidiabetic activity. Among all organic fractions, ethyl acetate exhibited strong antimicrobial potential followed by -hexane and chloroform fractions against selected pathogenic bacteria. Various concentrations of the ethyl acetate extract were tested for cytotoxicity and results indicated minor morphological changes in liver cells including ballooning, fatty droplets, and slight accumulation of extracellular matrix even at concentrations of 400 mg/kg. study showed that stigmasta-7,16-dien-3-ol had a strong interaction with COX-1 and COX-2 to reduce inflammation. The abovementioned results indicate the pharmacological strengths of to fight several diseases.
药用植物因其巨大的药理和免疫支持潜力而被广泛开发利用。[植物名称]的果实含有多种活性次生代谢产物，如酚类、黄酮类和精油，这些物质在传统医学中用作抗糖尿病、抗炎、抗氧化和抗菌剂。在本研究中，通过傅里叶变换红外光谱（FT-IR）、高效液相色谱（HPLC）和气相色谱-质谱联用（GC-MS）分析，对[植物名称]甲醇提取物的有机馏分（正己烷、氯仿和乙酸乙酯）中的植物成分进行了分析和鉴定。乙酸乙酯馏分在浓度为3mg/mL时表现出最高的抗氧化清除活性（76±0.769%）和抗炎活性（40±0.473%）。同样，通过抑制α-淀粉酶来测定抗糖尿病作用，其中乙酸乙酯馏分（77±0.844%）表现出最高的抗糖尿病活性。在所有有机馏分中，乙酸乙酯对所选病原菌表现出较强的抗菌潜力，其次是正己烷和氯仿馏分。对不同浓度的乙酸乙酯提取物进行了细胞毒性测试，结果表明，即使在浓度为400mg/kg时，肝细胞也出现了轻微的形态变化，包括气球样变、脂肪滴和细胞外基质的轻微积聚。[另一项]研究表明，豆甾-7,16-二烯-3-醇与COX-1和COX-2有很强的相互作用以减轻炎症。上述结果表明了[植物名称]对抗多种疾病的药理优势。
