State Key Laboratory of Frigid Zone Cardiovascular Diseases (SKLFZCD), College of Pharmacy, Harbin Medical University, Baojian Road No. 157, Harbin 150081, Heilongjiang, China.
College of Public Health, Harbin Medical University, Baojian Road No. 157, Harbin 150081, Heilongjiang, China.
ACS Appl Mater Interfaces. 2023 Jul 26;15(29):34419-34426. doi: 10.1021/acsami.3c04285. Epub 2023 Jul 12.
The conventional detection methods cannot satisfy the need for early and rapid detection of monkeypox virus (MPXV) infection. This is due to complicated pretreatment, time consumption, and complex operation of the diagnostic tests. Based on surface-enhanced Raman spectroscopy (SERS), this study attempted to capture the characteristic fingerprints of the MPXV genome and multiple antigenic proteins without the need to design specific probes. The minimum detection limit of this method is 100 copies/mL, with good reproducibility and signal-to-noise ratio. Therefore, the relationship between characteristic peak intensity and the protein and nucleic acid concentration can be used to construct a concentration-dependent spectral line with a good linear relationship. Additionally, principal component analysis (PCA) could identify the SERS spectra of four different MPXV proteins in serum. Therefore, this rapid detection method in the current outbreak of monkeypox control and the future response to possible new outbreaks has broad application prospects.
传统的检测方法无法满足对猴痘病毒（MPXV）感染进行早期和快速检测的需求。这是因为诊断测试的预处理复杂、耗时且操作复杂。本研究基于表面增强拉曼光谱（SERS），试图在无需设计特定探针的情况下捕捉 MPXV 基因组和多种抗原蛋白的特征指纹。该方法的最低检测限为 100 拷贝/mL，具有良好的重现性和信噪比。因此，可以使用特征峰强度与蛋白质和核酸浓度之间的关系来构建具有良好线性关系的浓度依赖性谱线。此外，主成分分析（PCA）可以识别血清中四种不同 MPXV 蛋白的 SERS 光谱。因此，这种在当前猴痘爆发控制和未来应对可能的新爆发中的快速检测方法具有广阔的应用前景。
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