College of Animal Science and Technology, Gansu Agricultural University, Lanzhou, 730070, China.
Farmer Education and Training Work Station of Gansu province, Lanzhou, 730070, China.
Vet Res Commun. 2023 Sep;47(3):1177-1184. doi: 10.1007/s11259-023-10118-w. Epub 2023 Jul 12.
Intestinal infection with C. perfringens is responsible for outbreaks of diarrhea in piglets. Janus kinase / signal transducer and activator of transcription (JAK/STAT) is a vital signaling pathway that regulates cellular activity and inflammatory response, closely correlated with multiple diseases development and advances. Currently, the potential effect of JAK/STAT on C. perfringens beta2 (CPB2) treatment on porcine intestinal epithelial (IPEC-J2) cells has not been explored. The expression of JAK/STAT genes or proteins in IPEC-J2 cells induced by CPB2 were observed by qRT-PCR and Western blot, and further used WP1066 to explore the effect of JAK2/STAT3 on mechanism employed by CPB2 on apoptosis, cytotoxicity, oxidative stress and inflammatory cytokines of IPEC-J2 cells. JAK2, JAK3, STAT1, STAT3, STAT5A and STAT6 were highly expressed in CPB2-induced IPEC-J2 cells, among which STAT3 had the highest expression. Moreover, apoptosis, cytotoxicity and oxidative stress were attenuated via blocking the activation of JAK2/STAT3 by using WP1066 in CPB2-treated IPEC-J2 cells. Furthermore, WP1066 significantly suppressed the secretion of interleukin (IL)-6, IL-1β and TNF-α induced by CPB2 in IPEC-J2 cells.Our findings provide some insights into the functional roles of JAK2/STAT3 in piglets against to C. perfringens infection.
产气荚膜梭菌引起的肠道感染可导致仔猪腹泻暴发。Janus 激酶/信号转导子和转录激活子（JAK/STAT）是一种重要的信号通路，调节细胞活性和炎症反应，与多种疾病的发生和进展密切相关。目前，尚未探讨 JAK/STAT 对产气荚膜梭菌β2（CPB2）治疗猪肠上皮（IPEC-J2）细胞的潜在影响。通过 qRT-PCR 和 Western blot 观察 CPB2 诱导的 IPEC-J2 细胞中 JAK/STAT 基因或蛋白的表达，并进一步使用 WP1066 探讨 JAK2/STAT3 对 CPB2 诱导的 IPEC-J2 细胞凋亡、细胞毒性、氧化应激和炎症细胞因子的作用机制。CPB2 诱导的 IPEC-J2 细胞中 JAK2、JAK3、STAT1、STAT3、STAT5A 和 STAT6 表达较高，其中 STAT3 表达最高。此外，通过 WP1066 阻断 JAK2/STAT3 的激活，可减轻 CPB2 处理的 IPEC-J2 细胞中的凋亡、细胞毒性和氧化应激。此外，WP1066 显著抑制 CPB2 诱导的 IPEC-J2 细胞中白细胞介素（IL）-6、IL-1β 和 TNF-α 的分泌。我们的研究结果为 JAK2/STAT3 在仔猪抵抗产气荚膜梭菌感染中的功能作用提供了一些见解。
