BIO@SNS Lab, Scuola Normale Superiore, 56126 Pisa, Italy.
Institute of Information Science and Technologies (ISTI-CNR), 56124 Pisa, Italy.
Cell Rep. 2023 Jul 25;42(7):112788. doi: 10.1016/j.celrep.2023.112788. Epub 2023 Jul 11.
Perineuronal nets (PNNs) surround specific neurons in the brain and are involved in various forms of plasticity and clinical conditions. However, our understanding of the PNN role in these phenomena is limited by the lack of highly quantitative maps of PNN distribution and association with specific cell types. Here, we present a comprehensive atlas of Wisteria floribunda agglutinin (WFA)-positive PNNs and colocalization with parvalbumin (PV) cells for over 600 regions of the adult mouse brain. Data analysis shows that PV expression is a good predictor of PNN aggregation. In the cortex, PNNs are dramatically enriched in layer 4 of all primary sensory areas in correlation with thalamocortical input density, and their distribution mirrors intracortical connectivity patterns. Gene expression analysis identifies many PNN-correlated genes. Strikingly, PNN-anticorrelated transcripts are enriched in synaptic plasticity genes, generalizing PNNs' role as circuit stability factors.
周围神经毡（PNNs）围绕着大脑中的特定神经元，并参与各种形式的可塑性和临床状况。然而，由于缺乏对 PNN 分布与特定细胞类型关联的高度定量图谱，我们对 PNN 在这些现象中的作用的理解是有限的。在这里，我们呈现了一个成年小鼠大脑超过 600 个区域的 Wisteria floribunda agglutinin（WFA）阳性 PNNs 及其与 parvalbumin（PV）细胞共定位的综合图谱。数据分析表明，PV 表达是 PNN 聚集的良好预测因子。在皮层中，PNNs 在所有初级感觉区域的 4 层中显著富集，与丘脑皮质输入密度相关，其分布反映了皮层内连接模式。基因表达分析确定了许多与 PNN 相关的基因。引人注目的是，与 PNN 相关的转录本在突触可塑性基因中富集，这概括了 PNN 作为回路稳定性因素的作用。
