Key Laboratory of Xin'an Medicine, Ministry of Education, Anhui University of Chinese Medicine, Hefei, Anhui, 230038, P. R. China.
Institute of Biomedical Engineering, the Second Clinical Medical College (Shenzhen People's Hospital) of Jinan University, Shenzhen, Guangdong, 518020, P. R. China.
Adv Healthc Mater. 2023 Nov;12(28):e2301370. doi: 10.1002/adhm.202301370. Epub 2023 Jul 21.
Continuous oxidative stress and cellular dysfunction caused by hyperglycemia are distinguishing features of diabetic wounds. It has been a great challenge to develop a smart dressing that can accelerate diabetic wound healing through regulating abnormal microenvironments. In this study, a platelet rich plasma (PRP) loaded multifunctional hydrogel with reactive oxygen species (ROS) and glucose dual-responsive property is reported. It can be conveniently prepared with PRP, dopamine (DA) grafted alginate (Alg-DA), and 6-aminobenzo[c][1,2]oxaborol-1(3H)-ol (ABO) conjugated hyaluronic acid (HA-ABO) through ionic crosslinks, hydrogen-bond interactions, and boronate ester bonds. The hydrogel possesses injectability, moldability, tissue adhesion, self-healing, low hemolysis, and hemostasis performances. Its excellent antioxidant property can create a low oxidative stress microenvironment for other biological events. Under an oxidative stress and/or hyperglycemia state, the hydrogel can degrade at an accelerated rate to release a variety of cytokines derived from activated blood platelets. The result is a series of positive changes that are favorable for diabetic wound healing, including fast anti-inflammation, activated macrophage polarization toward M2 phenotype, promoted migration and proliferation of fibroblasts, as well as expedited angiogenesis. This work provides an efficient strategy for chronic diabetic wound management and offers an alternative for developing a new-type PRP-based bioactive wound dressing.
高血糖引起的持续氧化应激和细胞功能障碍是糖尿病伤口的显著特征。开发一种智能敷料，通过调节异常的微环境来加速糖尿病伤口愈合，一直是一个巨大的挑战。在本研究中，报道了一种血小板富血浆（PRP）负载的具有活性氧（ROS）和葡萄糖双重响应性的多功能水凝胶。它可以方便地通过 PRP、多巴胺（DA）接枝海藻酸钠（Alg-DA）和硼酸酯键共轭透明质酸（HA-ABO）之间的离子交联、氢键相互作用和硼酸盐酯键制备。水凝胶具有可注射性、可模塑性、组织粘附性、自修复性、低溶血和止血性能。其优异的抗氧化性能可以为其他生物事件创造一个低氧化应激的微环境。在氧化应激和/或高血糖状态下，水凝胶可以加速降解，释放各种来自激活血小板的细胞因子。结果是一系列有利于糖尿病伤口愈合的积极变化，包括快速抗炎、巨噬细胞向 M2 表型的激活极化、成纤维细胞的迁移和增殖的促进以及血管生成的加速。这项工作为慢性糖尿病伤口管理提供了一种有效的策略，并为开发新型基于 PRP 的生物活性伤口敷料提供了一种替代方案。
