Department of Animal Sciences, University of Florida, Gainesville, FL, USA.
Department of Animal Sciences, University of Florida, Gainesville, FL, USA; Department of Animal and Dairy Sciences and Brown Loam Experiment Station, Mississippi State University, Raymond, MS, USA.
Theriogenology. 2023 Oct 1;209:226-233. doi: 10.1016/j.theriogenology.2023.07.003. Epub 2023 Jul 5.
The expression of interferon (IFN) stimulated genes (ISGs) in lymphocytes has been used for pregnancy diagnosis in cattle. However, among-cow variability has yielded sub-optimal predictive accuracy. We hypothesized that the expression of ISGs (ISG15, OAS1, RSAD2, CLEC3B, and AKR1B1) in early pregnancy varies according to the proportion of Bos indicus (B. indicus) genetics on females. Multiparous cows were classified in three genetic groups, High Angus (HA; n = 45 [0-33% Brahman influence]), Angus-Brahman (AB; n = 30 [34-67%]), and High Brahman (HB; n = 19 [68-100%]) and submitted to a Select-Synch + CIDR protocol. Cows that displayed estrus (n = 94) were artificially inseminated (Day0; D0). On D19, blood samples were collected to obtain peripheral blood mononuclear cells (PBMC) and measure progesterone (P4) concentrations. On D30, pregnancy diagnosis was performed. The expression of RSAD2 in PBMC of pregnant cows was positively related to the proportion of B. indicus genetics of the groups, but not the expression of ISG15 and OAS1. In pregnant cows, the proportion of B. indicus genetics was negatively associated to circulating levels of P4 concentrations. The P4 concentrations were related positively with RSAD2 expression. ROC curve results determined that for cattle with B. indicus genetics lower than 67%, the CLEC3B and AKR1B1 combination was the most accurate option to predict the outcome of pregnancy. In cows with more than 68% of B. indicus genetics, RSAD2 provided the best accuracy. In conclusion, there is a relationship between the proportion of B. indicus genetics and the ISGs gene expression in PBMC during pregnancy.
干扰素（IFN）刺激基因（ISGs）在淋巴细胞中的表达已被用于牛的妊娠诊断。然而，牛之间的变异性导致预测准确性不理想。我们假设，在怀孕早期，ISGs（ISG15、OAS1、RSAD2、CLEC3B 和 AKR1B1）的表达因母牛的印度野牛（B. indicus）遗传比例而异。经产奶牛被分为三个遗传群体，高安格斯（HA；n = 45 [0-33% Brahman 影响]）、安格斯-婆罗门（AB；n = 30 [34-67%]）和高婆罗门（HB；n = 19 [68-100%]），并接受选择同步+CIDR 方案。表现发情的奶牛（n = 94）进行人工授精（D0）。在 D19 天，采集血样以获得外周血单核细胞（PBMC）并测量孕酮（P4）浓度。在 D30 天，进行妊娠诊断。怀孕奶牛 PBMC 中 RSAD2 的表达与群体中印度野牛遗传比例呈正相关，但与 ISG15 和 OAS1 的表达无关。在怀孕奶牛中，印度野牛遗传比例与循环 P4 浓度呈负相关。P4 浓度与 RSAD2 表达呈正相关。ROC 曲线结果表明，对于印度野牛遗传比例低于 67%的牛，CLEC3B 和 AKR1B1 组合是预测妊娠结局的最准确选择。对于印度野牛遗传比例高于 68%的牛，RSAD2 提供了最佳的准确性。总之，在怀孕期间，PBMC 中的印度野牛遗传比例与 ISGs 基因表达之间存在关系。
