New Mexico State University, 1780 E University Ave, Las Cruces, NM, 88003, USA.
New Mexico State University, 1780 E University Ave, Las Cruces, NM, 88003, USA.
Differentiation. 2023 Sep-Oct;133:12-24. doi: 10.1016/j.diff.2023.06.001. Epub 2023 Jun 27.
The Drosophila melanogaster eye has been instrumental for determining both how cells communicate with one another to determine cell fate, as well as cell morphogenesis and patterning. Here, we describe the effects of the small GTPase Rap1 on the development of multiple cell types in the D. melanogaster eye. Although Rap1 has previously been linked to RTK-Ras-MAPK signaling in eye development, we demonstrate that manipulation of Rap1 activity is modified by increase or decrease of Delta/Notch signaling during several events of cell fate specification in eye development. In addition, we demonstrate that manipulating Rap1 function either in primary pigment cells or in interommatidial cells affects cone cell contact switching, primary pigment cell enwrapment of the ommatidial cluster, and sorting of secondary and tertiary pigment cells. These data suggest that Rap1 has roles in both ommatidial cell recruitment/survival and in ommatidial morphogenesis in the pupal stage. They lay groundwork for future experiments on the role of Rap1 in these events.
黑腹果蝇的眼睛在确定细胞之间如何通过相互通讯来决定细胞命运，以及细胞形态发生和模式形成方面发挥了重要作用。在这里，我们描述了小分子 GTPase Rap1 对黑腹果蝇眼睛中多种细胞类型发育的影响。尽管 Rap1 先前与 RTK-Ras-MAPK 信号通路在眼睛发育过程中有关，但我们证明，在眼睛发育过程中几个细胞命运特化事件中，Rap1 活性的操纵受到 Delta/Notch 信号通路的增加或减少的影响。此外，我们还证明，在初级色素细胞或细胞间细胞中操纵 Rap1 功能会影响锥体细胞接触转换、初级色素细胞对小眼簇的包裹以及次级和三级色素细胞的分选。这些数据表明 Rap1 在蛹期的小眼募集/存活和小眼形态发生中都有作用。它们为 Rap1 在这些事件中的作用的未来实验奠定了基础。
