Mezl V A, Fournier L A, Garber P M
Int J Biochem. 1986;18(8):705-11. doi: 10.1016/0020-711x(86)90393-9.
At optimum magnesium, the translation of rat heart mRNA in the nuclease treated rabbit reticulocyte lysate system was inhibited by low concentrations of spermidine or spermine but not of putrescine. Spermidine and spermine cause a general reduction in the translation of all the heart mRNAs since no differential effects were observed when the translation products were examined by gel electrophoresis. Spermine was a five times more potent inhibitor than spermidine but no inhibition was obtained with N1-acetylspermidine or N1-acetylspermine. Since analyses of endogenous polyamines demonstrate that the inhibitory concentrations of spermine could be obtained by converting a small fraction of the endogenous spermidine to spermine, these results indicate that interconversions of the polyamines might be a sensitive regulatory mechanism for protein synthesis.
在最佳镁离子浓度下，在经核酸酶处理的兔网织红细胞裂解物系统中，大鼠心脏mRNA的翻译受到低浓度亚精胺或精胺的抑制，但不受腐胺的抑制。亚精胺和精胺会导致所有心脏mRNA的翻译普遍减少，因为当通过凝胶电泳检查翻译产物时未观察到差异效应。精胺的抑制作用比亚精胺强五倍，但N1-乙酰亚精胺或N1-乙酰精胺未产生抑制作用。由于对内源性多胺的分析表明，通过将一小部分内源性亚精胺转化为精胺可以获得精胺的抑制浓度，这些结果表明多胺的相互转化可能是蛋白质合成的一种敏感调节机制。
