Murtiashaw M H, Winterhalter K H
Diabetologia. 1986 Jun;29(6):366-70. doi: 10.1007/BF00903346.
The study described here makes use of a new technique to assess the level of non-enzymatic glycosylation (glycation) by purified radioactively labelled glucose. Glycation up to 3 molglc/mol protein of human serum albumin, in contrast to previous reports, did not affect the binding of up to 2 mol palmitate, which was reached at a ratio of 7.5 palmitate/HSA. The uptake of palmitate from albumin by either erythrocytes or HL-60 cells also was not influenced by glycation of 3 mol glucose/mol protein. The distribution of palmitate into neutral lipids, phospholipids, or the palmitate designated for oxidation was likewise not influenced. This suggests that levels of albumin glycation likely to be encountered in the blood of diabetic subjects (up to 1 molglc/mol HSA) do not affect fatty acid utilization.
本文所述研究采用一种新技术，通过纯化的放射性标记葡萄糖来评估非酶糖基化（糖化）水平。与之前的报道相反，人血清白蛋白糖化至3摩尔葡萄糖/摩尔蛋白质时，并不影响高达2摩尔棕榈酸酯的结合，该结合在棕榈酸酯/人血清白蛋白比例为7.5时达到。红细胞或HL-60细胞从白蛋白摄取棕榈酸酯的过程同样不受3摩尔葡萄糖/摩尔蛋白质糖化的影响。棕榈酸酯在中性脂质、磷脂或用于氧化的棕榈酸酯中的分布同样不受影响。这表明糖尿病患者血液中可能出现的白蛋白糖化水平（高达1摩尔葡萄糖/摩尔人血清白蛋白）不会影响脂肪酸的利用。
