Department of Forensic Science, Virginia Commonwealth University, Richmond, VA, USA.
Methods Mol Biol. 2023;2685:35-51. doi: 10.1007/978-1-0716-3295-6_3.
There are several silica-based extraction methods that utilize silica-packed columns or silica-coated paramagnetic resin and are suitable for the needs of forensic DNA analysis and/or human identification. These rely on the use of chaotropic salts to alter the affinity of DNA such that it binds strongly to silica. A variety of samples can be successfully processed with these procedures, including buccal swabs, dried or liquid blood, saliva, semen, and other typical forensic-type samples. This chapter will describe the manual extraction process for Promega's DNA™ IQ System, as well as Qiagen's QIAamp DNA Blood Mini Kit, QIAamp DNA Mini Kit, and QIAamp DNA Investigator Kit.
有几种基于硅的提取方法，使用硅填充柱或硅涂层顺磁树脂，适用于法医 DNA 分析和/或人类识别的需求。这些方法依赖于使用离液盐来改变 DNA 的亲和力，使其与硅强烈结合。这些程序可以成功处理各种样本，包括口腔拭子、干燥或液体血液、唾液、精液和其他典型的法医样本。本章将描述 Promega 的 DNA IQ 系统以及 Qiagen 的 QIAamp DNA 血液迷你试剂盒、QIAamp DNA 迷你试剂盒和 QIAamp DNA Investigator 试剂盒的手动提取过程。
