Applied Biosystems 的 GlobalFiler™ PCR 扩增试剂盒。
Applied Biosystems' GlobalFiler™ PCR Amplification Kit.
机构信息
Forensic Science Program, George Mason University, Fairfax, VA, USA.
Department of Forensic Sciences, The George Washington University, Washington, DC, USA.
出版信息
Methods Mol Biol. 2023;2685:241-252. doi: 10.1007/978-1-0716-3295-6_15.
The GlobalFiler™ PCR Amplification Kit is one of the most sensitive kits that exist today that makes the PCR amplification of human DNA possible. PCR amplification using this specific kit makes millions of copies of 24 specific target sequences in the DNA, called markers or loci. This kit is a 6-dye, short tandem repeat (STR) multiplex assay kit that has a synthetic mix of primers and single-stranded oligonucleotides that are combined with DNA samples and then subjected to 29 or 30 cycles of denaturing, annealing, and extension, as per laboratory protocol. Methods for instrument operation will vary depending on the thermal cycler instrument model that is used. Nevertheless, the GlobalFiler™ PCR Amplification Kit has proven to be a very useful tool to DNA analysts, amplifying extremely low quantities of DNA, making it possible to detect partial, if not full, genetic profiles from a wide range of sample types. This chapter discusses the typical preparation and PCR amplification of human forensic DNA samples, using the GlobalFiler™ PCR Amplification Kit.
全球filer PCR 扩增试剂盒是当今最灵敏的试剂盒之一，可实现人类 DNA 的 PCR 扩增。使用该特定试剂盒进行 PCR 扩增，可使 DNA 中 24 个特定目标序列（称为标记物或基因座）的拷贝数增加数百万倍。该试剂盒是一种 6 色、短串联重复（STR）多重分析试剂盒，包含合成的引物混合物和单链寡核苷酸，与 DNA 样本结合，然后根据实验室方案进行 29 或 30 个循环的变性、退火和延伸。仪器操作方法因使用的热循环仪型号而异。然而，全球filer PCR 扩增试剂盒已被证明是 DNA 分析人员非常有用的工具，可扩增极低量的 DNA，从而能够从广泛的样本类型中检测到部分（即使不是全部）遗传谱。本章讨论了使用全球filer PCR 扩增试剂盒对人类法医 DNA 样本进行的典型制备和 PCR 扩增。
Zotero 插件