He Wenting, Gan Yijia, Qi Xingyi, Wang Han, Song Hanyue, Su Ping, Song Jiayi, Yang Yi
Beijing Key Laboratory of Environmentally Harmful Chemical Analysis, College of Chemistry, Beijing University of Chemical Technology, Beijing 100029, P.R. China.
ACS Appl Mater Interfaces. 2023 Jul 26;15(29):34513-34526. doi: 10.1021/acsami.3c05237. Epub 2023 Jul 13.
Enzyme immobilization enables the fabrication of flexible and powerful biocatalytic systems that can meet the needs of green and efficient development in various fields. However, restricted electron and mass transfer during enzymatic reactions and disruption of the enzyme structure during encapsulation limit the wide application of the immobilized enzyme systems. Herein, we report an encapsulation strategy based on hollow-shell-layered double hydroxides (LDHs; ZnCo-LDH) for green and nondestructive enzyme immobilization. Benefiting from the protective and enzyme-friendly microenvironment provided by the hydrophilic hollow structure of ZnCo-LDH, the encapsulated enzyme maintains a nearly natural enzyme biostructure and enhanced stability. Notably, mesoporous ZnCo-LDH with excellent electrical properties considerably facilitates electron and mass transport during enzymatic reactions, exhibiting 5.56 times the catalytic efficiency of free enzymes or traditional enzyme encapsulation systems. The current study broadens the family of encapsulated carriers and alleviates the trade-off between enzyme stability and catalytic activity in the encapsulated state, presenting a promising avenue for the industrial application of the enzyme.
酶固定化能够构建灵活且强大的生物催化系统，满足各个领域绿色高效发展的需求。然而，酶促反应过程中受限的电子和质量传递以及包封过程中酶结构的破坏限制了固定化酶系统的广泛应用。在此，我们报道了一种基于中空壳层状双氢氧化物（LDHs；ZnCo-LDH）的包封策略，用于绿色且无损的酶固定化。得益于ZnCo-LDH亲水性中空结构提供的保护且对酶友好的微环境，被包封的酶保持了近乎天然的酶生物结构并提高了稳定性。值得注意的是，具有优异电学性质的介孔ZnCo-LDH极大地促进了酶促反应过程中的电子和质量传输，其催化效率是游离酶或传统酶包封系统的5.56倍。当前的研究拓宽了包封载体家族，并缓解了包封状态下酶稳定性和催化活性之间的权衡，为酶的工业应用提供了一条有前景的途径。
