Bose Susmita, Koski Caitlin, Bhattacharjee Arjak
W. M. Keck Biomedical Materials Research Laboratory, School of Mechanical and Materials Engineering, Washington State University, Pullman, WA 99164, USA.
J Mater Res. 2022 Jun;37(12):2033-2044. doi: 10.1557/s43578-022-00602-5. Epub 2022 Jun 15.
The possibilities of utilizing nacre as a reinforcing material to manufacture 3D printed bone grafts are yet to be explored. This work reports the feasibility of fabricating 3D printed nacre-hydroxyapatitestarch composite bone graft substitutes, emphasizing the effects of nacre addition on biological and mechanical properties. Pressure-less extrusion-based 3D printing of ceramic-polymer viscous slurry is challenging due to the composition and process-parameter variations. To overcome these challenges, a dual extrusion solid freeform fabricator (SFF) has been designed. An increase in nacre loading improves the compressive strength from 9.5 ± 0.1 MPa to 11.7 ± 0.2 MPa, without any post-processing or sintering. Nacre's in vitro osteogenic properties lead to a slight increase in hFOB cellular attachment on the graft surface by day 11. The fabricated structures show good mechanical integrity during the dissolution study in simulated body fluid (SBF). These bone graft substitutes may be utilized to repair low load bearing skeletal defects.
利用珍珠母作为增强材料制造3D打印骨移植体的可能性尚未得到探索。这项工作报告了制造3D打印珍珠母-羟基磷灰石-淀粉复合骨移植替代物的可行性，强调了添加珍珠母对生物学和力学性能的影响。由于成分和工艺参数的变化，基于无压挤压的陶瓷-聚合物粘性浆料3D打印具有挑战性。为了克服这些挑战，设计了一种双挤压固体自由成型制造机（SFF）。在没有任何后处理或烧结的情况下，珍珠母负载量的增加将抗压强度从9.5±0.1MPa提高到11.7±0.2MPa。珍珠母的体外成骨特性导致在第11天时，人永生化表皮角质形成细胞（hFOB）在移植体表面的附着略有增加。在模拟体液（SBF）中的溶解研究期间，制造的结构显示出良好的机械完整性。这些骨移植替代物可用于修复低负荷骨骼缺损。
