Selavka C M, Krull I S, Lurie I S
Forensic Sci Int. 1986 Jun;31(2):103-17. doi: 10.1016/0379-0738(86)90194-5.
Electrochemical detection in HPLC offers a very sensitive and selective alternative to UV detection for a number of classes of compounds. While there have been no reports of the use of electrochemical detection for the determination of cocaine in illicit preparations or biological fluids, it is now possible to use on-line, continuous post-column photolytic derivatization to generate electroactive species from cocaine which may then be detected downstream using a conventional electrochemical detector operated at oxidative working electrode potentials. In this manuscript the construction, optimization and characterization of system parameters are discussed, and the sensitive and highly selective nature of this novel detection method is demonstrated to be uniquely suited to the rapid identification and quantitation of cocaine in simulated illicit preparations.
在高效液相色谱法中，电化学检测为许多类化合物提供了一种比紫外检测更为灵敏和具选择性的方法。虽然目前尚无关于在非法制剂或生物流体中使用电化学检测来测定可卡因的报道，但现在可以采用在线、连续的柱后光解衍生化方法，从可卡因生成电活性物质，然后使用在氧化工作电极电位下操作的传统电化学检测器在下游进行检测。本文讨论了该系统参数的构建、优化和表征，并证明了这种新型检测方法的灵敏性和高选择性，特别适合于快速鉴定和定量模拟非法制剂中的可卡因。
