Department of Obstetrics and Gynecology, The First Affiliated Hospital of Soochow University, Suzhou 215006, Jiangsu, China; Department of Obstetrics and Gynecology, The Second Hospital of Nanjing, Nanjing University of Chinese Medicine, Nanjing 210003, Jiangsu, China.
Department of Gynecology, Nanjing Hospital of Chinese Medicine Affiliated to Nanjing University of Chinese Medicine, Nanjing 210022, Jiangsu, China.
Toxicology. 2023 Aug 1;494:153592. doi: 10.1016/j.tox.2023.153592. Epub 2023 Jul 11.
Beta-cypermethrin (β-CYP) is a commonly used insecticide that is potentially toxic and has adverse effects on the health of both animals and humans. Studies have indicated that β-CYP damages organs like the liver, thyroid, intestinal tract, and uterus. However, the underlying mechanisms that β-CYP affects oocyte quality are poorly understood. According to our research, β-CYP exposure led to the aberrant assembly of spindles and alignment of chromosomes, resulting in porcine oocytes' defective nuclear maturation. Concurrently, β-CYP exposure perturbed the cytoplasmic maturation by disturbing the cortical granules (CGs), endoplasmic reticulum (ER), and mitochondrial integrity. It also led to accumulating reactive oxygen species (ROS) and apoptosis. We found that supplementation with glutathione (GSH) mitigated the meiotic defects induced by β-CYP exposure via regulating ROS levels. Our observations illustrate that β-CYP exposure adversely impacts oocyte meiotic maturation, and taking GSH supplementation is an effective strategy.
β-氯氰菊酯（β-CYP）是一种常用的杀虫剂，具有潜在毒性，对动物和人类健康有不良影响。研究表明，β-CYP 会损害肝脏、甲状腺、肠道和子宫等器官。然而，β-CYP 影响卵母细胞质量的潜在机制尚不清楚。根据我们的研究，β-CYP 暴露导致纺锤体异常组装和染色体排列，导致猪卵母细胞核成熟缺陷。同时，β-CYP 暴露通过扰乱皮质颗粒（CGs）、内质网（ER）和线粒体完整性来干扰细胞质成熟。它还导致活性氧（ROS）和细胞凋亡的积累。我们发现，用谷胱甘肽（GSH）补充可以通过调节 ROS 水平来减轻β-CYP 暴露引起的减数分裂缺陷。我们的观察表明，β-CYP 暴露对卵母细胞减数分裂成熟有不利影响，而补充 GSH 是一种有效的策略。
