Sahraeian Samira, Abbaszadeh Hojjat Allah, Taheripanah Robabeh, Edalatmanesh Mohammad Amin, Keshavarzi Somayeh, Ghazifard Alaleh
Department of Biology, College of Sciences, Shiraz Branch, Islamic Azad University, Shiraz, Iran.
Laser Applications in Medical Sciences Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
J Lasers Med Sci. 2024 Apr 30;15:e10. doi: 10.34172/jlms.2024.10. eCollection 2024.
Polycystic ovary syndrome (PCOS) is a complex condition that can have various symptoms and complications, one of which is infertility. Dysregulation of miRNA has been associated with the pathogenesis of numerous illnesses such as PCOS. In this study, we evaluated the effect of photobiomodulation therapy (PBMT) and exosome therapy (EXO) on the regulation of miRNA and nucleus acetylation in a PCOS oocyte. In this research, 36 oocytes divided into three groups: control, EXO, and PBM (Wavelength of 640 nm). Subsequently, in-vitro maturation (IVM) was evaluated. Real-time PCR was used to evaluate and . Afterward, oocyte glutathione (GSH) and nucleus acetylation were measured by H4K12. The expression of the and genes in the EXO and PBMT groups was significantly down-regulated in comparison to the control group, but the expression of and significantly increased in the EXO and PBMT groups in comparison to the control group. The EXO and PBMT significantly increased GSH and nucleus acetylation (<0.0001). The results of this study showed that the use of EXO and PBMT can improve GSH and nucleus acetylation in the PCOS oocyte and also change the expression of miRNAs.
多囊卵巢综合征（PCOS）是一种复杂的病症，会出现各种症状和并发症，其中之一就是不孕。微小RNA（miRNA）失调与包括PCOS在内的多种疾病的发病机制有关。在本研究中，我们评估了光生物调节疗法（PBMT）和外泌体疗法（EXO）对PCOS卵母细胞中miRNA调节和细胞核乙酰化的影响。在本研究中，36个卵母细胞被分为三组：对照组、EXO组和PBM组（波长640nm）。随后，评估体外成熟（IVM）情况。采用实时定量聚合酶链反应（Real-time PCR）来评估[此处原文缺失具体评估内容]。之后，通过H4K12测量卵母细胞谷胱甘肽（GSH）和细胞核乙酰化情况。与对照组相比，EXO组和PBMT组中[此处原文缺失具体基因名称]基因的表达显著下调，但与对照组相比，EXO组和PBMT组中[此处原文缺失具体基因名称]的表达显著增加。EXO和PBMT显著增加了GSH和细胞核乙酰化水平（<0.0001）。本研究结果表明，使用EXO和PBMT可改善PCOS卵母细胞中的GSH和细胞核乙酰化水平，还可改变miRNA的表达。
