Chongqing Center for Disease Control and Prevention, 400042, Chongqing, China.
National Center for AIDS/STD Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 102206, China.
Virol J. 2023 Jul 13;20(1):147. doi: 10.1186/s12985-023-02112-0.
Over the past few years, HIV transmission among men who have sex with men (MSM) in China has increased significantly. Chongqing, located in the southwest of China, has the highest prevalence of HIV among MSM in the country.
Blood samples were taken from 894 MSM in Chongqing who had recently been diagnosed with HIV-1 infection and had not yet started getting treatment. In order to determine the distribution of HIV-1 subtypes, transmitted drug resistance, and assessments of molecularly transmitted clusters, we sequenced the Pol genes and employed them in phylogenetic analysis. The genetic distance between molecular clusters was 1.5%. To find potential contributing factors, logistic regression analyses were performed.
Of the 894 HIV-1 pol sequences acquired from study participants, we discovered that CRF07_BC (73.6%) and CRF01_AE (19.6%) were the two most prevalent HIV-1 genotypes in Chongqing among MSM, accounting for 93.2% of all infections. In addition, CRF08_BC (1.1%), B subtype (1.0%), CRF55_01B (3.4%), and URF/Other subtypes (1.3%) were less frequently observed. Among MSM in Chongqing, transmitted drug resistance (TDR) was reported to be present at a rate of 5.6%. 48 clusters with 600 (67.1%, 600/894) sequences were found by analysis of the molecular transmission network. The distributions of people by age, sexual orientation, syphilis, and genotype were significantly differentially related to being in clusters, according to the multivariable logistic regression model.
Despite the low overall prevalence of TDR, the significance of genotypic drug resistance monitoring needs to be emphasized. CRF07_BC and CRF01_AE were the two main genotypes that created intricate molecular transmission networks. In order to prevent the expansion of molecular networks and stop the virus's spread among MSM in Chongqing, more effective HIV intervention plans should be introduced.
过去几年，中国男男性行为者（MSM）中的 HIV 传播显著增加。中国西南部的重庆是全国 MSM 中 HIV 感染率最高的地区。
从最近被诊断出 HIV-1 感染且尚未开始接受治疗的 894 名重庆 MSM 中采集血液样本。为了确定 HIV-1 亚型的分布、传播耐药性以及分子传播簇的评估，我们对 Pol 基因进行了测序，并进行了系统发育分析。分子簇之间的遗传距离为 1.5%。为了寻找潜在的促成因素，我们进行了逻辑回归分析。
从研究参与者中获得的 894 个 HIV-1 pol 序列中，我们发现 CRF07_BC（73.6%）和 CRF01_AE（19.6%）是重庆 MSM 中两种最常见的 HIV-1 基因型，占所有感染的 93.2%。此外，还观察到 CRF08_BC（1.1%）、B 亚型（1.0%）、CRF55_01B（3.4%）和 URF/其他亚型（1.3%）的比例较低。在重庆的 MSM 中，报道的传播耐药性（TDR）率为 5.6%。通过分子传播网络分析发现了 48 个包含 600 个（894 个序列的 67.1%，600/894）序列的簇。多变量逻辑回归模型表明，年龄、性取向、梅毒和基因型分布与聚类之间存在显著差异。
尽管总体 TDR 流行率较低，但强调基因型耐药监测的重要性。CRF07_BC 和 CRF01_AE 是构成复杂分子传播网络的两种主要基因型。为了防止分子网络的扩大和阻止重庆 MSM 中的病毒传播，应引入更有效的 HIV 干预计划。
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