Key Laboratory of Functional Protein Research of Guangdong Higher Education Institutes and MOE Key Laboratory of Tumor Molecular Biology, Institute of Life and Health Engineering, Jinan University, Guangzhou, China.
Sino-French Hoffmann Institute, School of Basic Medical Sciences, State Key Laboratory of Respiratory Disease, Guangzhou Medical University, Guangzhou, China.
Sci Data. 2023 Jul 13;10(1):455. doi: 10.1038/s41597-023-02359-w.
The development of high-throughput omics technology has greatly promoted the development of biomedicine. However, the poor reproducibility of omics techniques limits their application. It is necessary to use standard reference materials of complex RNAs or proteins to test and calibrate the accuracy and reproducibility of omics workflows. The transcriptome and proteome of most cell lines shift during culturing, which limits their applicability as standard samples. In this study, we demonstrated that the human hepatocellular cell line MHCC97H has a very stable transcriptome (r = 0.9830.997) and proteome (r = 0.9660.988 for data-dependent acquisition, r = 0.970~0.994 for data-independent acquisition) after 9 subculturing generations, which allows this steady standard sample to be consistently produced on an industrial scale in long term. Moreover, this stability was maintained across labs and platforms. In sum, our study provides omics standard reference material and reference datasets for transcriptomic and proteomics research. This helps to further standardize the workflow and data quality of omics techniques and thus promotes the application of omics technology in precision medicine.
高通量组学技术的发展极大地推动了生物医学的发展。然而，组学技术的可重复性差限制了其应用。有必要使用复杂 RNA 或蛋白质的标准参考物质来测试和校准组学工作流程的准确性和可重复性。大多数细胞系的转录组和蛋白质组在培养过程中发生变化，这限制了它们作为标准样本的适用性。在这项研究中，我们证明了人肝癌细胞系 MHCC97H 在经过 9 次传代培养后具有非常稳定的转录组（r=0.9830.997）和蛋白质组（数据依赖采集 r=0.9660.988，数据独立采集 r=0.970~0.994），这使得这种稳定的标准样品能够在长期内以工业规模稳定生产。此外，这种稳定性在不同实验室和平台之间得以保持。总之，我们的研究为转录组学和蛋白质组学研究提供了组学标准参考物质和参考数据集。这有助于进一步规范组学技术的工作流程和数据质量，从而促进组学技术在精准医学中的应用。
