Caton A J
Hybridoma. 1986 Jul;5 Suppl 1:S11-6.
Sequence analysis of murine monoclonal antibodies (MAbs) specific for the CO17-1A antigen is being performed to assess the structural basis for antibody specificity for this antigen. Preliminary data reveal that the heavy chain variable regions of MAbs GA733 and CO17-1A (designated Ab1s since they were isolated following immunization with CO17-1A antigen) most likely utilize distinct members of the same gene family. Remarkably, they also display identical amino acid sequences in their heavy chain CDR3 regions, reflecting absolute nucleotide sequence identity across their VH-D-JH junctions. Since these junctional sequences are randomly generated during the assembly of immunoglobulin genes and in general display enormous diversity, this homology suggests an important role for heavy chain CDR3 in determining specificity for the CO17-1A antigen in these antibodies.
正在对针对CO17-1A抗原的鼠单克隆抗体（MAb）进行序列分析，以评估抗体针对该抗原的特异性的结构基础。初步数据显示，MAb GA733和CO17-1A的重链可变区（由于它们是在用CO17-1A抗原免疫后分离得到的，因此称为Ab1）很可能利用了同一基因家族的不同成员。值得注意的是，它们在重链CDR3区域也显示出相同的氨基酸序列，这反映了它们的VH-D-JH连接区的绝对核苷酸序列同一性。由于这些连接序列是在免疫球蛋白基因组装过程中随机产生的，并且通常显示出巨大的多样性，这种同源性表明重链CDR3在决定这些抗体对CO17-1A抗原的特异性方面起着重要作用。
