Kolasinac Sabina Sibcic, Moe Lars, Rootwelt Vibeke, Sørum Henning
Department of Companion Animal Clinical Sciences, Faculty of Veterinary Medicine, Norwegian University of Life Sciences, Elizabeth Stephansens vei 15, 1433 Ås, Norway.
Department of Paraclinical Sciences, Faculty of Veterinary Medicine, Norwegian University of Life Sciences, Elizabeth Stephansens vei 15, 1433 Ås, Norway.
Animals (Basel). 2023 Jul 5;13(13):2206. doi: 10.3390/ani13132206.
Studies of microbiota in normal canine milk from healthy dams are sparse. As is the case with blood and urine, it was considered that milk contains no microbiota. Any discovery of bacteria in canine milk is, therefore, often noted to be a result of contamination during sampling or interpreted as mastitis and treated with antibiotics. Milk was collected twice within 19 days after natural parturition from 11 lactating dams, with no general or local clinical signs of mastitis or other disease. The skin and teats were prepared with an antimicrobial protocol prior to each milk sampling. In total, 210 milk samples were collected and assessed for a number of bacterial colonies grown on each plate. Bacterial growth was detected in 180 samples (86%). , spp., spp., Coagulase-Negative Staphylococci (CoNS), spp., , spp., spp., and were identified from pure and/or mixed bacterial growth, listed in descending order of occurrence. Despite the small sample size, the consistent occurrence of bacteria in early postpartum dams indicates a genuine occurrence of bacteria in canine milk, rather than random contamination. The finding of bacteria in the milk of dams should not, therefore, be the sole argument for the diagnosis of mastitis.
关于健康母犬正常乳汁中微生物群的研究很少。与血液和尿液的情况一样，人们认为乳汁中不存在微生物群。因此，在犬乳中发现的任何细菌通常都被认为是采样过程中污染的结果，或者被解释为乳腺炎并用抗生素治疗。在自然分娩后的19天内，从11只泌乳母犬身上采集了两次乳汁，这些母犬没有乳腺炎或其他疾病的全身或局部临床症状。每次采集乳汁前，都按照抗菌方案对皮肤和乳头进行处理。总共采集了210份乳汁样本，并评估了每个平板上生长的细菌菌落数量。在180个样本（86%）中检测到细菌生长。从纯培养和/或混合细菌生长中鉴定出了 属、 属、凝固酶阴性葡萄球菌（CoNS）、 属、 属、 属、 属和 属，按出现频率从高到低列出。尽管样本量较小，但产后早期母犬乳汁中细菌的持续出现表明犬乳中确实存在细菌，而不是随机污染。因此，在母犬乳汁中发现细菌不应作为诊断乳腺炎的唯一依据。
