Mekkawy Ahmed H, Breakeit Mohammad, Pillai Krishna, Badar Samina, Akhter Javed, Valle Sarah J, Morris David L
Mucpharm Pty Ltd., Sydney, NSW 2217, Australia.
Department of Surgery, St George Hospital, Sydney, NSW 2217, Australia.
Cancers (Basel). 2023 Jun 24;15(13):3321. doi: 10.3390/cancers15133321.
A combination of bromelain and acetylcysteine, BromAc, is an efficient intraperitoneal mucolytic for thick mucus secreted in pseudomyxoma peritonei (PMP). Patients with PMP quite often undergo colon anastomosis. Hence, we investigated the effect of the intraperitoneal delivery of BromAc on colon-anastomosis healing in a rat model. Sixteen Wistar rats were divided into two groups (N = 8). The controls received intraperitoneal saline after anastomosis, whilst the other group received BromAc. They were monitored for body-weight and general health parameters. Half the rats in each group (N = 4) were culled at 4 or 13 days post-surgery for assessment. The healing process of the tissues was assessed by burst pressure and collagen density with histology to assess the integrity of the internal organs. The results indicated that there was a similar pattern of weight fluctuation during the experiment, although the rats treated with the BromAc showed slightly greater weight loss during the first 4 days. Although the burst pressure was similar in both groups, the BromAc group at day 13 showed a slightly higher burst pressure, which was complemented by a higher collagen density (albeit not statistically significant). The histology of the internal organs was comparable to those of the controls. This study indicates that the intraperitoneal delivery of BromAc in a rat model does not interfere with the healing process of colonic anastomosis.
菠萝蛋白酶和乙酰半胱氨酸的组合制剂BromAc，是一种有效的腹膜内黏液溶解剂，可用于溶解腹膜假黏液瘤（PMP）分泌的浓稠黏液。PMP患者经常需要进行结肠吻合术。因此，我们在大鼠模型中研究了腹腔注射BromAc对结肠吻合口愈合的影响。将16只Wistar大鼠分为两组（每组n = 8）。对照组在吻合术后接受腹腔注射生理盐水，而另一组接受BromAc注射。监测它们的体重和一般健康参数。每组中的一半大鼠（n = 4）在术后4天或13天被处死以进行评估。通过爆破压力和胶原蛋白密度评估组织的愈合过程，并通过组织学评估内部器官的完整性。结果表明，在实验过程中两组大鼠体重波动模式相似，尽管接受BromAc治疗的大鼠在最初4天内体重下降略多。虽然两组的爆破压力相似，但BromAc组在第13天的爆破压力略高，同时胶原蛋白密度也较高（尽管无统计学意义）。内部器官的组织学与对照组相当。本研究表明，在大鼠模型中腹腔注射BromAc不会干扰结肠吻合口的愈合过程。
