Sobieraj Karolina, Stegenta-Dąbrowska Sylwia, Zafiu Christian, Binner Erwin, Białowiec Andrzej
Department of Applied Bioeconomy, Wrocław University of Environmental and Life Sciences, 37a Chełmońskiego Str., 51-630 Wrocław, Poland.
Department of Water-Atmosphere-Environment, Institute of Waste Management and Circularity, University of Natural Resources and Life Sciences, Muthgasse 107, 1190 Vienna, Austria.
Materials (Basel). 2023 Jun 23;16(13):4551. doi: 10.3390/ma16134551.
Despite the development of biorefinery processes, the possibility of coupling the "conventional" composting process with the production of biochemicals is not taken into account. However, net carbon monoxide (CO) production has been observed during bio-waste composting. So far, O concentration and temperature have been identified as the main variables influencing CO formation. This study aimed to investigate CO net production during bio-waste composting under controlled laboratory conditions by varying aeration rates and temperatures. A series of composting processes was carried out in conditions ranging from mesophilic to thermophilic (T = 35, 45, 55, and 65 °C) and an aeration rate of 2.7, 3.4, 4.8, and 7.8 L·h. Based on the findings of this study, suggestions for the improvement of CO production throughout the composting process have been developed for the first time. The highest concentrations of CO in each thermal variant was achieved with an O deficit (aeration rate 2.7 L·h); additionally, CO levels increased with temperature, reaching ~300 ppm at 65 °C. The production of CO in mesophilic and thermophilic conditions draws attention to biological CO formation by microorganisms capable of producing the CODH enzyme. Further research on CO production efficiency in these thermal ranges is necessary with the characterization of the microbial community and analysis of the ability of the identified bacteria to produce the CODH enzyme and convert CO from CO.
尽管生物炼制工艺有所发展，但“传统”堆肥过程与生物化学品生产相结合的可能性却未被考虑在内。然而，在生物废弃物堆肥过程中已观察到有一氧化碳（CO）净生成。到目前为止，氧气浓度和温度已被确定为影响CO形成的主要变量。本研究旨在通过改变曝气速率和温度，在可控的实验室条件下研究生物废弃物堆肥过程中的CO净生成情况。在从嗜温到嗜热（T = 35、45、55和65°C）以及曝气速率为2.7、3.4、4.8和7.8 L·h的条件下进行了一系列堆肥过程。基于本研究的结果，首次针对整个堆肥过程中CO生成的改善提出了建议。在每个温度变体中，当氧气不足（曝气速率2.7 L·h）时，CO浓度最高；此外，CO水平随温度升高而增加，在65°C时达到约300 ppm。嗜温和嗜热条件下CO的生成引起了人们对能够产生CODH酶的微生物进行生物CO形成的关注。有必要在这些温度范围内进一步研究CO的生产效率，并对微生物群落进行表征，分析已鉴定细菌产生CODH酶以及将CO转化为CO的能力。
