Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán, A.C., Calle 43 No. 130 x 32 y 34, Chuburná de Hidalgo, Mérida C.P. 97205, Yucatán, Mexico.
Unidad de Biotecnología, Centro de Investigación Científica de Yucatán, A.C., Calle 43 No. 130 x 32 y 34, Chuburná de Hidalgo, Mérida C.P. 97205, Yucatán, Mexico.
Int J Mol Sci. 2023 Jun 21;24(13):10431. doi: 10.3390/ijms241310431.
L. is a crop grown in the humid tropics. It is grouped into two classes of varieties: dwarf and tall; regardless of the variety, the endosperm of the coconut accumulates carbohydrates in the early stages of maturation and fatty acids in the later stages, although the biochemical factors that determine such behavior remain unknown. We used tandem mass tagging with synchronous precursor selection (TMT-SPS-MS3) to analyze the proteomes of solid endosperms from Yucatan green dwarf (YGD) and Mexican pacific tall (MPT) coconut cultivars. The analysis was conducted at immature, intermediate, and mature development stages to better understand the regulation of carbohydrate and lipid metabolisms. Proteomic analyses showed 244 proteins in YGD and 347 in MPT; from these, 155 proteins were shared between both cultivars. Furthermore, the proteomes related to glycolysis, photosynthesis, and gluconeogenesis, and those associated with the biosynthesis and elongation of fatty acids, were up-accumulated in the solid endosperm of MPT, while in YGD, they were down-accumulated. These results support that carbohydrate and fatty acid metabolisms differ among the developmental stages of the solid endosperm and between the dwarf and tall cultivars. This is the first proteomics study comparing different stages of maturity in two contrasting coconut cultivars and may help in understanding the maturity process in other palms.
椰子是一种生长在湿润热带地区的作物。它分为两个品种：矮种和高种；无论品种如何，椰子的胚乳在成熟的早期积累碳水化合物，在后期积累脂肪酸，尽管决定这种行为的生化因素尚不清楚。我们使用串联质量标记与同步前体选择（TMT-SPS-MS3）来分析尤卡坦绿矮（YGD）和墨西哥太平洋高（MPT）椰子品种的固体胚乳的蛋白质组。分析在不成熟、中等和成熟发育阶段进行，以更好地理解碳水化合物和脂质代谢的调节。蛋白质组学分析显示，YGD 中有 244 种蛋白质，MPT 中有 347 种蛋白质；其中，155 种蛋白质在这两个品种中是共有的。此外，与糖酵解、光合作用和糖异生相关的蛋白质组，以及与脂肪酸生物合成和伸长相关的蛋白质组，在 MPT 的固体胚乳中积累较多，而在 YGD 中则积累较少。这些结果表明，碳水化合物和脂肪酸代谢在固体胚乳的发育阶段以及矮种和高种之间存在差异。这是首次对两个不同椰子品种在不同成熟阶段进行蛋白质组学比较的研究，可能有助于理解其他掌类植物的成熟过程。
