Zhou Zhongjing, Lin Baogang, Tan Jinjuan, Hao Pengfei, Hua Shuijin, Deng Zhiping
State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou, China.
Zhejiang Key Laboratory of Digital Dry Land Crops, Institute of Crops and Nuclear Technology Utilization, Zhejiang Academy of Agricultural Sciences, Hangzhou, China.
Front Plant Sci. 2022 Jun 2;13:907244. doi: 10.3389/fpls.2022.907244. eCollection 2022.
Enhancing oil content is one of the major goals in breeding; however, genetic regulation of seed oil content in plants is complex and not fully elucidated. In this study, we report proteins that were differentially accumulated in immature seeds of 35 days after anthesis between two recombinant inbred lines with contrasting seed oil content, high oil content line (HOCL) and low oil content line (LOCL) using a multiplex isobaric tandem mass tags (TMT)-based quantitative proteomic approach. Over 4,600 proteins were quantified in seeds of the two lines, and 342 proteins showed differential accumulation between seeds of HOCL and LOCL. Gene Ontology enrichment analysis revealed that the differentially accumulated proteins were enriched in proteins involved in lipid biosynthesis and metabolism, photosynthesis, and nutrient reservoir activity. Western blot confirmed the increased abundance of a late embryogenesis abundant protein (BnLEA57) in HOCL seeds compared with LOCL seeds, and overexpression of either gene or its homology in transgenic enhanced oil content in seeds. Our work provides new insights into the molecular regulatory mechanism of seed oil content in .
提高油含量是育种的主要目标之一；然而，植物种子油含量的遗传调控很复杂，尚未完全阐明。在本研究中，我们使用基于多重等压串联质量标签（TMT）的定量蛋白质组学方法，报道了在开花后35天的未成熟种子中差异积累的蛋白质，这些种子来自两个种子油含量相反的重组自交系，即高油含量系（HOCL）和低油含量系（LOCL）。在两个系的种子中对超过4600种蛋白质进行了定量，并且342种蛋白质在HOCL和LOCL的种子之间显示出差异积累。基因本体富集分析表明，差异积累的蛋白质富集于参与脂质生物合成和代谢、光合作用以及营养储存活性的蛋白质中。蛋白质免疫印迹证实，与LOCL种子相比，HOCL种子中一种晚期胚胎发生丰富蛋白（BnLEA57）的丰度增加，并且在转基因植物中该基因或其同源基因的过表达提高了种子中的油含量。我们的工作为[植物名称未给出]种子油含量的分子调控机制提供了新的见解。
