Key Laboratory of Bioresource Research and Development of Liaoning Province, College of Life and Health Sciences, Northeastern University, Shenyang 110169, China.
Liaoning Province Outstanding Innovation Team, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China.
Int J Mol Sci. 2023 Jul 3;24(13):11020. doi: 10.3390/ijms241311020.
The understanding of the molecular defensive mechanism of (L.) Moench against polycyclic aromatic hydrocarbon (PAH) contamination plays a key role in the further improvement of phytoremediation efficiency. Here, the responses of to a defined mixture of phenanthrene (PHE) and pyrene (PYR) at different concentrations or a natural mixture from an oilfield site with a history of several decades were studied based on transcriptomics sequencing and widely targeted metabolomics approaches. The results showed that upon 60-day PAH exposure, the growth of in terms of biomass ( < 0.01) and leaf area per plant ( < 0.05) was negatively correlated with total PAH concentration and significantly reduced at high PAH level. The majority of genes were switched on and metabolites were accumulated after exposure to PHE + PYR, but a larger set of genes (3964) or metabolites (208) showed a response to a natural PAH mixture in . The expression of genes involved in the pathways, such as chlorophyll cycle and degradation, circadian rhythm, jasmonic acid signaling, and starch and sucrose metabolism, was remarkably regulated, enhancing the ability of to adapt to PAH exposure. Tightly associated with transcriptional regulation, metabolites mainly including sugars and secondary metabolites, especially those produced via the phenylpropanoid pathway, such as coumarins, flavonoids, and their derivatives, were increased to fortify the adaptation of to PAH contamination. These results suggest that has a positive defense mechanism against PAHs, which opens new avenues for the research of phytoremediation mechanism and improvement of phytoremediation efficiency via a mechanism-based strategy.
对 （L.）Moench 抵御多环芳烃（PAH）污染的分子防御机制的理解在进一步提高植物修复效率方面起着关键作用。在这里，基于转录组测序和广泛靶向代谢组学方法，研究了 对定义的菲（PHE）和芘（PYR）混合物或来自具有数十年历史的油田的天然混合物在不同浓度下的响应。结果表明，在 60 天 PAH 暴露下， （ < 0.01）和每株植物的叶面积（ < 0.05）的生物量生长与总 PAH 浓度呈负相关，在高 PAH 水平下显著降低。大多数基因在暴露于 PHE + PYR 后被开启并积累代谢物，但在 中，更多的基因（3964 个）或代谢物（208 个）对天然 PAH 混合物有反应。涉及途径的基因表达，如叶绿素循环和降解、昼夜节律、茉莉酸信号和淀粉和蔗糖代谢，受到显著调节，增强了 适应 PAH 暴露的能力。与转录调节紧密相关，代谢物主要包括糖和次生代谢物，特别是那些通过苯丙烷途径产生的，如香豆素、类黄酮及其衍生物，增加了以加强 对 PAH 污染的适应。这些结果表明， 对 PAHs 具有积极的防御机制，为植物修复机制的研究和通过基于机制的策略提高植物修复效率开辟了新途径。
