短间隔重复盒式序列（SIRC）-在.中发现的新型重复 DNA 元件。

Short Interrupted Repeat Cassette (SIRC)-Novel Type of Repetitive DNA Element Found in .

机构信息

Cell Biology and Bioengineering, Siberian Institute of Plant Physiology and Biochemistry SB RAS, Irkutsk 664033, Russia.

Department of Business Communications and Informatics, Irkutsk State University, Irkutsk 664033, Russia.

出版信息

Int J Mol Sci. 2023 Jul 5;24(13):11116. doi: 10.3390/ijms241311116.

DOI:10.3390/ijms241311116

PMID:37446293

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10342631/

Abstract

Short interrupted repeat cassette (SIRC)-a novel DNA element found throughout the nuclear genome. SIRCs are represented by short direct repeats interrupted by diverse DNA sequences. The maxima of SIRC's distribution are located within pericentromeric regions. We suggest that originally SIRC was a special case of the complex internal structure of the miniature inverted repeat transposable element (MITE), and further MITE amplification, transposition, and loss of terminal inverted repeats gave rise to SIRC as an independent DNA element. SIRC sites were significantly enriched with several histone modifications associated with constitutive heterochromatin and mobile genetic elements. The majority of DNA-binding proteins, strongly associated with SIRC, are related to histone modifications for transcription repression. A part of SIRC was found to overlap highly inducible protein-coding genes, suggesting a possible regulatory role for these elements, yet their definitive functions need further investigation.

摘要

短间隔重复盒（SIRC）-一种存在于整个核基因组中的新型 DNA 元件。SIRCs 由短的直接重复序列组成，被不同的 DNA 序列所打断。SIRC 的分布最大值位于着丝粒周围区域。我们推测，最初 SIRC 是微型反向重复转座元件（MITE）复杂内部结构的一个特殊情况，进一步的 MITE 扩增、转座和末端反向重复的丢失导致 SIRC 作为一个独立的 DNA 元件出现。SIRC 位点与几种与组成型异染色质和移动遗传元件相关的组蛋白修饰显著富集。与 SIRC 强烈相关的大多数 DNA 结合蛋白与转录抑制的组蛋白修饰有关。发现一部分 SIRC 与高度诱导的蛋白质编码基因重叠，表明这些元件可能具有调节作用，但它们的明确功能仍需进一步研究。