Sección de Estudios de Postgrado, Escuela Superior de Medicina, Instituto Politécnico Nacional, Ciudad de México 11340, Mexico.
Departamento de Biología Celular, Centro de Investigación y de Estudios Avanzados del IPN (CINVESTAV), Av. IPN No. 2508 Col. San Pedro Zacatenco, Ciudad de México 07360, Mexico.
Int J Mol Sci. 2023 Jul 7;24(13):11216. doi: 10.3390/ijms241311216.
() is a parasite in humans that provokes amoebiasis. The most employed drug is metronidazole (MTZ); however, some studies have reported that this drug induces genotoxic effects. Therefore, it is necessary to explore new compounds without toxicity that can eliminate . Flavonoids are polyphenolic compounds that have demonstrated inhibition of growth and dysregulation of amoebic proteins. Despite the knowledge acquired to date, action mechanisms are not completely understood. The present work evaluates the effect of kaempferol against trophozoites and in the interactions with neutrophils from hamster, which is a susceptibility model. Our study demonstrated a significant reduction in the amoebic viability of trophozoites incubated with kaempferol at 150 μM for 90 min. The gene expression analysis showed a significant downregulation of Pr (peroxiredoxin), Rr (rubrerythrin), and TrxR (thioredoxin reductase). In interactions with amoebae and neutrophils for short times, we observed a reduction in ROS (reactive oxygen species), NO (nitric oxide), and MPO (myeloperoxidase) neutrophil activities. In conclusion, we confirmed that kaempferol is an effective drug against through the decrease in antioxidant enzyme expression and a regulator of several neutrophil mechanisms, such as MPO activity and the regulation of ROS and NO.
（）是一种寄生在人类体内的寄生虫，可引起阿米巴病。最常用的药物是甲硝唑（MTZ）；然而，一些研究报告称，这种药物会引起遗传毒性作用。因此，有必要探索没有毒性的新化合物来消灭。类黄酮是一种多酚化合物，已被证明可以抑制生长和调节阿米巴蛋白。尽管迄今为止已经获得了相关知识，但作用机制仍不完全清楚。本工作评估了山奈酚对滋养体的作用以及与仓鼠中性粒细胞的相互作用，仓鼠是一种易感模型。我们的研究表明，在 150μM 下孵育 90 分钟后，滋养体的存活能力显著降低。基因表达分析显示，Pr（过氧化物还原酶）、Rr（rubrerythrin）和 TrxR（硫氧还蛋白还原酶）的表达显著下调。在与阿米巴和中性粒细胞短时间相互作用的过程中，我们观察到 ROS（活性氧）、NO（一氧化氮）和 MPO（髓过氧化物酶）中性粒细胞活性的减少。综上所述，我们证实山奈酚通过降低抗氧化酶的表达和调节几种中性粒细胞机制，如 MPO 活性以及 ROS 和 NO 的调节，是一种有效的抗药。
