Kelly I D, Smith S
Int J Environ Anal Chem. 1986;25(1-3):135-49. doi: 10.1080/03067318608077083.
The metabolism of benazolin-ethyl (4-chloro-2-oxobenzothiazolin-3-ylacetic acid ethyl ester), a post emergence herbicide, has been studied in soybean using (14C)-phenyl labelled compound. Preliminary studies were performed on excised soybean leaves. Following hydrolysis of the ethyl ester to benazolin acid (4-chloro-2-oxobenzothiazolin-3-ylacetic acid), extensive metabolism to polar conjugates was observed. The polar fraction from a Bligh-Dyer extraction was purified by solvent partitioning, preparative TLC and reverse phase HPLC with ion suppression. The two major metabolites were characterised by fast atom bombardment mass spectrometry with accurate mass determination as an aspartate conjugate and a malonyl-beta-glucose ester of benazolin acid. Subsequent experiments were performed by spraying intact plants at growth stage V4. The major polar metabolite isolated one month after treatment was identified as the aspartate conjugate by mass spectrometry and high resolution nuclear magnetic resonance spectroscopy.
使用（14C）-苯基标记化合物，对芽后除草剂乙基苯唑灵（4-氯-2-氧代苯并噻唑啉-3-基乙酸乙酯）在大豆中的代谢进行了研究。在离体大豆叶片上进行了初步研究。乙酯水解为苯唑灵酸（4-氯-2-氧代苯并噻唑啉-3-基乙酸）后，观察到大量代谢为极性共轭物。通过溶剂分配、制备型薄层色谱和离子抑制反相高效液相色谱对布莱-戴尔萃取法得到的极性部分进行了纯化。通过快速原子轰击质谱法和精确质量测定，鉴定出两种主要代谢物为苯唑灵酸的天冬氨酸共轭物和丙二酰-β-葡萄糖酯。随后在V4生长阶段对完整植株进行喷雾处理实验。处理后一个月分离得到的主要极性代谢物通过质谱法和高分辨率核磁共振光谱法鉴定为天冬氨酸共轭物。
