Department of Pharmaceutics, Semmelweis University, Hőgyes Str. 7, 1092 Budapest, Hungary.
Department of Biophysics and Radiation Biology, Semmelweis University, Tűzoltó Str. 37-7, 1094 Budapest, Hungary.
Molecules. 2023 Jun 29;28(13):5125. doi: 10.3390/molecules28135125.
Knowledge of the physical and chemical properties of phospholipids, such as phase transition temperatures (Tc), is of great importance in order to reveal the functionalities of biological and artificial membranes. Our research group developed an oscillatory rheological method for the simple and rapid determination of phase transition temperatures (Tc). The phospholipids constructing the membranes undergo conformational changes at their Tc, which cause alterations of viscoelastic properties of the molecules. The oscillatory technique recommended by us proved to be appropriate to reveal the altered molecular properties of phospholipids as tracking the slightest changes in the viscoelasticity. Our study demonstrates the abrupt changes in rheological properties at Tc for the following phospholipids: 1,2-Dimyristoyl-sn-glycero-3-Phosphocholine (DMPC), 1,2-Dipalmitoyl-sn-glycero-3-Phosphatidylcholine (DPPC), and 1,2-Distearoyl-sn-glycero-3-Phosphocholine (DSPC), proving that the applied methodology is adequate for determining the Tc of phospholipids.
了解磷脂的物理和化学性质，如相变温度（Tc），对于揭示生物和人工膜的功能非常重要。我们的研究小组开发了一种振荡流变学方法，用于简单快速地测定相变温度（Tc）。构成膜的磷脂在 Tc 时会发生构象变化，这会导致分子的粘弹性发生变化。我们推荐的振荡技术被证明适合揭示磷脂分子性质的变化，因为它可以跟踪粘弹性的最微小变化。我们的研究表明，以下磷脂在 Tc 时流变性质会发生突然变化：1,2-二肉豆蔻酰-sn-甘油-3-磷酸胆碱（DMPC）、1,2-二月桂酰-sn-甘油-3-磷脂酰胆碱（DPPC）和 1,2-二月桂酰-sn-甘油-3-磷酸胆碱（DSPC），证明所应用的方法适用于测定磷脂的 Tc。
