Ingeniería de Procesos Sustentables, Facultad Regional San Francisco, Universidad Tecnológica Nacional, San Francisco 2400, Argentina.
Unidad de Investigación y Desarrollo en Tecnología Farmacéutica (UNITEFA), CONICET, Córdoba 5000, Argentina.
Molecules. 2023 Jul 7;28(13):5286. doi: 10.3390/molecules28135286.
Sweet basil ( leaves are rich in bioactive compounds that present therapeutic benefits for human health. Ultrasonic-assisted extraction (UAE) is frequently used to obtain phenolic compounds from plants/herbal sources. However, few works have developed multi-variable studies to find the optimal conditions to extract the maximum amount of compounds, especially when applied to UAE via a sonotrode. The purpose of this work was to perform a multi-variable study by employing a Box-Behnken design to collect the highest active compound content from leaves. The efficacy of the design was endorsed by ANOVA. The studied parameters for UAE via a sonotrode were the ethanol/water ratio, amplitude, and time. The analyzed responses were the rosmarinic acid, the sum of phenolic acids, and the sum of phenolic compounds content. The optimal conditions were found to be 50% ethanol/water, 50% amplitude, and 5 min. Twenty bioactive compounds were identified by HPLC-ESI-TOF-MS when the extract was collected by applying the optimal conditions. may be appreciated as a valuable source of important bioactive substances for pharmaceutical use.
甜罗勒（ Ocimum basilicum L.）的叶子富含生物活性化合物，对人类健康具有治疗益处。超声辅助提取（ UAE）常用于从植物/草药来源中提取酚类化合物。然而，很少有研究进行多变量研究以找到提取最大化合物量的最佳条件，特别是当应用于通过超声变幅杆的 UAE 时。这项工作的目的是通过使用 Box-Behnken 设计进行多变量研究，从 叶子中收集最高含量的活性化合物。方差分析（ ANOVA）支持了设计的有效性。通过超声变幅杆进行 UAE 的研究参数为乙醇/水比、振幅和时间。分析的响应为迷迭香酸、总酚酸和总酚类化合物含量。当应用最佳条件收集提取物时，发现最佳条件为 50%乙醇/水、50%振幅和 5 分钟。通过 HPLC-ESI-TOF-MS 鉴定了 20 种生物活性化合物。 可能被视为用于药物用途的重要生物活性物质的有价值来源。
