Centro de Investigação de Montanha (CIMO), Instituto Politécnico de Bragança, Campus de Santa Apolónia, 5300-253 Bragança, Portugal.
Nutrition and Bromatology Group, Department of Analytical and Food Chemistry, Faculty of Food Science and Technology, University of Vigo-Ourense Campus, E-32004 Ourense, Spain.
Molecules. 2019 Feb 14;24(4):686. doi: 10.3390/molecules24040686.
Heat-Assisted Extraction (HAE) was used for the optimized production of an extract rich in anthocyanin compounds from var. leaves. The optimization was performed using the response surface methodology employing a central composite experimental design with five-levels for each of the assessed variables. The independent variables studied were the extraction time (, 20⁻120 min), temperature (, 25⁻85 °C), and solvent (, 0⁻100% of ethanol, ). Anthocyanin compounds were analysed by HPLC-DAD-ESI/MS and the extraction yields were used as response variables. Theoretical models were developed for the obtained experimental data, then the models were validated by a selected number of statistical tests, and finally, those models were used in the prediction and optimization steps. The optimal HAE conditions for the extraction of anthocyanin compounds were: = 65.37 ± 3.62 min, = 85.00 ± 1.17 °C and = 62.50 ± 4.24%, and originated 114.74 ± 0.58 TA mg/g of extract. This study highlighted the red rubin basil leaves as a promising natural matrix to extract pigmented compounds, using green solvents and reduced extraction times. The extract rich in anthocyanins also showed antimicrobial and anti-proliferative properties against four human tumor cell lines, without any toxicity on a primary porcine liver cell line.
热辅助提取 (HAE) 用于从 var. 叶中优化生产富含花色苷化合物的提取物。使用响应面法，采用五水平的中心复合实验设计对提取时间 (, 20⁻120 min)、温度 (, 25⁻85 °C) 和溶剂 (, 0⁻100% 的乙醇,) 进行了优化。花色苷化合物通过 HPLC-DAD-ESI/MS 进行分析，提取产率用作响应变量。为获得的实验数据开发了理论模型，然后通过选择的统计测试对模型进行了验证，最后在预测和优化步骤中使用了这些模型。花色苷化合物提取的 HAE 最佳条件为： = 65.37 ± 3.62 min， = 85.00 ± 1.17 °C 和 = 62.50 ± 4.24%，提取物中含 114.74 ± 0.58 TA mg/g 的花色苷。本研究强调了红罗勒叶作为一种有前途的天然基质，可使用绿色溶剂和缩短提取时间来提取色素化合物。富含花色苷的提取物还表现出对四种人肿瘤细胞系的抗菌和抗增殖特性，对原代猪肝细胞系没有任何毒性。
